Phorbol esters potentiate the induction of class I HLA expression by interferon. alpha

Journal Article · Wed Mar 01 00:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.6.1973· OSTI ID:5030403

Erusalimsky, J D; Kefford, R F; Gilmore, D J; Milstein, C ^[1]

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1. Medical Research Council Laboratory of Molecular Biology, Cambridge (England)

The authors have studied the effect of phorbol esters on the induction of class I histocompatibility antigen (HLA) expression by interferons (IFNs) in the T-cell line MOLT-4 and in the MOLT-4 mutant YHHH. Addition of IFN-{alpha} to phorbol 12,13-dibutyrate-pretreated MOLT-4 cells causes a >20-fold increase in the expression of class I HLA, as compared to a 4- to 7-fold IFN-{alpha}-induced increase in control cells. Pretreatment with phorbol 12,13-dibutyrate does not alter the class I HLA response to IFN-{gamma} or the responses of other IFN-induced genes. This effect of phorbol 12,13-dibutyrate reproduces in MOLT-4 cells the phenotype of the mutant YHHH, which also displays a selective enhanced class I HLA response to IFN-{alpha}. Pretreatment of YHHH with phorbol 12,13-dibutyrate does not affect any of the responses induced by IFN. These findings suggest the existence of a phorbol ester-sensitive factor, inducible in MOLT-4 and constitutively expressed or modified in YHHH, which operates in the pathway of induction of class I HLA by IFN-{alpha} but not in the pathway used by IFN-{gamma}.

• OSTI ID: 5030403
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:6; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
INTERFERON
BIOCHEMISTRY
PHORBOL ESTERS
BIOLOGICAL EFFECTS
ANTIGENS
HISTOCOMPATIBILITY COMPLEX
LYMPHOCYTES
MAN
MESSENGER-RNA
TUMOR CELLS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CHEMISTRY
CONNECTIVE TISSUE CELLS
ESTERS
GROWTH FACTORS
LEUKOCYTES
LYMPHOKINES
MAMMALS
MATERIALS
MITOGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RNA
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology