Stearoyl-acyl-carrier-protein desaturase from higher plants is structurally unrelated to the animal and fungal homologs

Journal Article · Fri Mar 15 00:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.6.2510· OSTI ID:5030234

Shanklin, J; Somerville, C ^[1]

---

1. Michigan State Univ., East Lansing (United States)

Stearoyl-acyl-carrier-protein (ACP) desaturase was purified to homogeneity from avocado mesocarp, and monospecific polyclonal antibodies directed against the protein were used to isolate full-length cDNA clones from Ricinus communis (castor) seed and Cucumis sativus (cucumber). The nucleotide sequence of the castor clone pRCD1 revealed an open reading frame of 1.2 kilobases encoding a 396-amino acid protein of 45 kDa. The cucumber clone pCSD1 encoded a homologous 396-amino acid protein with 88% amino acid identity to the castor clone. Expression of pRCD1 in Saccharomyces cerevisiae resulted in the accumulation of a functional stearoyl-ACP desaturase, demonstrating that the introduction of this single gene product was sufficient to confer soluble desaturase activity to yeast. There was a 48-residue region of 29% amino acid sequence identity between residues 53 and 101 of the castor desaturase and the proximal border of the dehydratase region of the fatty acid synthase from yeast. Stearoyl-ACP mRNA was present at substantially higher levels in developing seeds than in leaf and root tissue, suggesting that expression of the {Delta}{sup 9} desaturase is developmentally regulated.

• DOE Contract Number: AC02-76ER01338
• OSTI ID: 5030234
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:6, Issue 6; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HYDROXYLASES
AMINO ACID SEQUENCE
RECOMBINANT DNA
DNA SEQUENCING
ANTIBODIES
BIOLOGICAL EVOLUTION
CASTOR
CHLOROPLASTS
CUCUMBERS
ELECTROPHORESIS
PHOSPHORUS 32
PLANT CELLS
SACCHAROMYCES CEREVISIAE
STEARATES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACID SALTS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DNA
ENZYMES
EUMYCOTA
EUPHORBIA
FOOD
FUNGI
ISOTOPES
LIGHT NUCLEI
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MEDICINAL PLANTS
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PHOSPHORUS ISOTOPES
PLANTS
RADIOISOTOPES
SACCHAROMYCES
STRUCTURAL CHEMICAL ANALYSIS
VEGETABLES
YEASTS
550201* - Biochemistry- Tracer Techniques