Defective disposal of immune complexes and polyclonal B cell activation persist long after exposure to bacterial lipopolysaccharide in mice

Journal Article · Wed Nov 01 00:00:00 EST 1989 · Laboratory Investigation; (USA)

OSTI ID:5023527

Granholm, N A; Cavallo, T ^[1]

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1. Rhode Island Hospital, Providence (USA)

Patients with systemic lupus erythematosus experience clinical exacerbation during superimposed bacterial infection. Previous studies in mice indicated that heightened immune phenomena during exposure to bacterial lipopolysaccharide (LPS) appear to be related, in part, to polyclonal B cell activation, to abnormal disposal of immune complexes (IC), and to increased localization of IC in tissues. To investigate whether such effects were reversible, we administered bacterial LPS to C57BL/6 mice for 5 weeks. Control mice received vehicle alone. We then discontinued LPS, and 6 weeks later LPS and control mice were challenged with a subsaturating dose of radiolabeled IC; the removal of IC from the circulation, their localization in the liver, spleen, and kidney were determined. In comparison to values in control mice, in mice previously exposed to LPS, serologic features of polyclonal B cell activation persisted; liver uptake of pathogenic IC (greater than Ag2Ab2) was normal, but removal of small size IC (less than or equal to Ag2Ab2) from the circulation was delayed; localization of IC in the kidneys was enhanced, and pathologic proteinuria developed. The effects of repeated exposure to bacterial LPS are partially reversible, but they last long after LPS is discontinued and may contribute to altered disposal of IC, enhanced organ localization of IC, and organ dysfunction.

• OSTI ID: 5023527
• Journal Information: Laboratory Investigation; (USA), Vol. 61:5; ISSN 0023-6837
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
IMMUNE SYSTEM DISEASES
PATHOGENESIS
LYMPHOCYTES
CELL PROLIFERATION
ANTIGEN-ANTIBODY REACTIONS
LIPOPOLYSACCHARIDES
LIVER
MICE
PATIENTS
RADIOISOTOPES
SALMONELLA
TISSUE DISTRIBUTION
TRACER TECHNIQUES
ANIMAL CELLS
ANIMALS
BACTERIA
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
DISEASES
DISTRIBUTION
GLANDS
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LIPIDS
MAMMALS
MATERIALS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANS
POLYSACCHARIDES
RODENTS
SACCHARIDES
SOMATIC CELLS
VERTEBRATES
550901* - Pathology- Tracer Techniques