Phosphatidylinositol(4,5)bisphosphate and phosphatidylinositol(4)phosphate in plant tissues. [Pisum sativum]

Journal Article · Wed Mar 01 00:00:00 EST 1989 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.89.3.888· OSTI ID:5023442

Irvine, R F; Letcher, A J; Lander, D J ^[1]; Dawson, A P ^[2]; Musgrave, A ^[3]; Drobak, B K ^[4]

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1. Institute of Animal Physiology and Genetics Research, Cambridge (England)
2. Univ. of East Anglia, Norwich (England)
3. Univ. of Amsterdam (Netherlands)
4. John Innes Institute, Norwich (England)

Pea (Pisum sativum) leaf discs or swimming suspensions of Chlamydomonas eugametos were radiolabeled with ({sup 3}H)myo-inositol or ({sup 32}P)Pi and the lipids were extracted, deacylated, and their glycerol moieties removed. The resulting inositol trisphosphate and bisphosphate fractions were examined by periodate degradation, reduction and dephosphorylation, or by incubation with human red cell membranes. Their likely structures were identified as D-myo-inositol(1,4,5)trisphosphate and D-myo-inositol(1,4,)-bisphosphate. It is concluded that plants contain phosphatidylinositol(4)phosphate and phosphatidylinositol(4,5)bisphosphate; no other polyphosphoinositides were detected.

• OSTI ID: 5023442
• Journal Information: Plant Physiology; (USA), Vol. 89:3; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LIPIDS
CHEMICAL COMPOSITION
PLANT TISSUES
PHYSIOLOGY
CELL MEMBRANES
CHLAMYDOMONAS
INOSITOLS
PHOSPHORUS
PHOSPHORUS 32
PISUM
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALGAE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CELL CONSTITUENTS
CHLOROPHYCOTA
DAYS LIVING RADIOISOTOPES
ELEMENTS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LEGUMINOSAE
LIGHT NUCLEI
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MEMBRANES
MICROORGANISMS
MONOSACCHARIDES
NONMETALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PLANTS
RADIOISOTOPES
SACCHARIDES
TISSUES
UNICELLULAR ALGAE
551001* - Physiological Systems- Tracer Techniques