Effects of peptides cleaved from human fibrinogen by plasmin on rabbit kidney cells in culture
Low molecular weight fibrinogen degradation products (LMW-FDP) containing a mixture of dialysable peptides cleaved from human fibrinogen by plasmin are cytotoxic to an established line of rabbit kidney cells and to primary cultures of rabbit kidney cells. The presence of LMW-FDP in a concentration of 50 micrograms/ml during the cell cultivation caused a considerable release of /sup 51/Cr from prelabelled cells and inhibited /sup 3/H-thymidine and /sup 86/Rb uptake. Among three isolated peptides of established primary structure only one, 6D: Ser-Gln-Leu-Gln-Lys-Val-Pro-Pro-Glu-Trp-Lys, induced a significant effect, i.e. it enhanced /sup 3/H-thymidine incorporation. Two others, 6A: Ala-Arg-Pro-Ala-Lys and 6E: Thr-Ser-Glu-Val-Lys, did not influence the examined parameters. Hence other components of LMW-FDP must be assumed to be responsible for the cytotoxic effect on kidney cell cultures.
- Research Organization:
- Inst. of Rheumatology, Warsaw, Poland
- OSTI ID:
- 5022339
- Journal Information:
- Thromb. Res.; (United States), Vol. 29:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHROMIUM 51
TRACER TECHNIQUES
PEPTIDES
TOXICITY
RUBIDIUM 86
SOMATIC CELLS
CELL KILLING
TRITIUM COMPOUNDS
FIBRINOGEN
FIBRINOLYSIN
IN VITRO
KIDNEYS
RABBITS
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD COAGULATION FACTORS
BODY
CHROMIUM ISOTOPES
COAGULANTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
FIBRINOLYTIC AGENTS
GLOBULINS
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PROTEINS
RADIOISOTOPES
RUBIDIUM ISOTOPES
SERINE PROTEINASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques