Affinity labeling of protein synthesis factors
Journal Article
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5022149
The authors laboratory is interested in determining those eukaryotic protein synthesis factors which interact with nucleotides and mRNA. To study the binding the authors have used the nucleotides, their analogs, and mRNA analogs as listed below: (1) UV cross-linking with normal (/sup 32/P)XTP; (2) Oxidized GTP; (3) 3'p-azido benzoyl GDP (GTP); (4) 5'p-fluoro sulfonyl benzoyl guanosine; (5) 5'p-fluoro sulfonyl benzoyl adenosine; (6) oxidized mRNA. Currently, they are continuing their efforts to specifically label the proteins, and they are also trying to isolate a single labeled tryptic peptide from the proteins.
- Research Organization:
- Case Western Reserve Univ., Cleveland, OH
- OSTI ID:
- 5022149
- Report Number(s):
- CONF-8606151-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
( sup 32 P)3 prime -O-(4-benzoyl)benzoyl ATP as a photoaffinity label for a phospholipase C-coupled P2Y-purinergic receptor
Solubilization of a guanine nucleotide-sensitive form of the P2Y-purinergic receptor
(5'-/sup 32/P)-8-azidoguanosine-3',5'-monophosphate. I. Synthesis and properties. II. Interaction with E. coli proteins
Journal Article
·
Wed Aug 15 00:00:00 EDT 1990
· Journal of Biological Chemistry; (USA)
·
OSTI ID:5022149
Solubilization of a guanine nucleotide-sensitive form of the P2Y-purinergic receptor
Journal Article
·
Mon Jul 01 00:00:00 EDT 1991
· Molecular Pharmacology; (United States)
·
OSTI ID:5022149
(5'-/sup 32/P)-8-azidoguanosine-3',5'-monophosphate. I. Synthesis and properties. II. Interaction with E. coli proteins
Thesis/Dissertation
·
Sat Jan 01 00:00:00 EST 1983
·
OSTI ID:5022149
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
PROTEINS
BIOCHEMICAL REACTION KINETICS
GENETIC RADIATION EFFECTS
LABELLING
AFFINITY
CROSS-LINKING
MESSENGER-RNA
NUCLEOTIDES
PHOSPHORUS 32
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
POLYMERIZATION
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
REACTION KINETICS
RNA
550201* - Biochemistry- Tracer Techniques
560111 - Radiation Effects on Biochemicals- In Vitro- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
PROTEINS
BIOCHEMICAL REACTION KINETICS
GENETIC RADIATION EFFECTS
LABELLING
AFFINITY
CROSS-LINKING
MESSENGER-RNA
NUCLEOTIDES
PHOSPHORUS 32
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
POLYMERIZATION
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
REACTION KINETICS
RNA
550201* - Biochemistry- Tracer Techniques
560111 - Radiation Effects on Biochemicals- In Vitro- (-1987)