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Title: Autoradiographic visualization of CNS receptors for vasoactive intestinal peptide

Receptors for VIP were characterized in the rat CNS. /sup 125/I-VIP bound with high affinity to rat brain slices. Binding was time dependent and specific. Pharmacology studies indicated that specific /sup 125/I-VIP binding was inhibited with high affinity by VIP and low affinity by secretin and PHI. Using in vitro autoradiographic techniques high grain densities were present in the dentate gyrus, pineal gland, supraoptic and suprachiasmatic nuclei, superficial gray layer of the superior colliculus and the area postrema. Moderate grain densities were present in the olfactory bulb and tubercle, cerebral cortex, nucleus accumbens, caudate putamen, interstitial nucleus of the stria terminalis, paraventricular thalamic nucleus, medial amygdaloid nucleus, subiculum and the medial geniculate nucleus. Grains were absent in the corpus callosum and controls treated with 1 microM unlabeled VIP. The discrete regional distribution of VIP receptors suggest that it may function as an important modulator of neural activity in the CNS.
Authors:
;
Publication Date:
OSTI Identifier:
5021554
Resource Type:
Journal Article
Resource Relation:
Journal Name: Peptides (Fayetteville, N.Y.); (United States); Journal Volume: 2
Research Org:
George Washington Univ. School of Medicine and Health Sciences, Washington, DC
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BRAIN; AUTORADIOGRAPHY; PEPTIDES; RADIORECEPTOR ASSAY; IODINE 125; LABELLED COMPOUNDS; RATS; RECEPTORS; SECRETIN; ANIMALS; BETA DECAY RADIOISOTOPES; BODY; CENTRAL NERVOUS SYSTEM; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MEMBRANE PROTEINS; NERVOUS SYSTEM; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOISOTOPES; RODENTS; TRACER TECHNIQUES; VERTEBRATES 550201* -- Biochemistry-- Tracer Techniques