Reinvestigation of the biosynthesis of 2-aminoethylphosphonic acid in Tetrahymena pyriformis

Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5020557

Barry, R J; Dunaway-Mariano, D; Mariano, P S

The initial step in the proposed biosynthetic pathway of 2-aminoethylphosphonate involves an intramolecular rearrangement of phosphoenolpyruvate to form the C-P compound, 3-phosphonopyruvate. A radioisotopic assay with authentic phosphonopyruvate as a cold carrier results in the formation of material chromatographically identical to phosphonopyruvate. NMR and degradation studies reveal that the assayed product is not p-pyr, but rather phosphoglyceric acid. Herein they report results from a reinvestigation of the biosynthesis of AEP in T. pyriformis. Recent study indicates that AEP is formed when radiolabeled Pi or PEP are used as precursors. P-pyr formation has yet to be demonstrated. Results from studies aimed at the direct verification or exclusion of p-pyr as an intermediate in the biosynthetic pathway leading to AEP formation is presented.

• Research Organization: Univ. of Maryland, College Park
• OSTI ID: 5020557
• Report Number(s): CONF-8606151-; TRN: 86-034810
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
PHOSPHONIC ACIDS
BIOSYNTHESIS
MOLECULAR STRUCTURE
BIOLOGICAL PATHWAYS
NMR SPECTRA
PRECURSOR
RADIOASSAY
TETRAHYMENA
ANIMALS
CILIATA
INVERTEBRATES
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PROTOZOA
SPECTRA
SYNTHESIS
550201* - Biochemistry- Tracer Techniques
550600 - Medicine