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Title: Studies on the organization and regeneration of bone marrow: origin, growth, and differentiation of endocloned hematopoietic colonies

Abstract

Hematopoietic colonies were studied by light microscopy in the marrow of alternate fraction x-irradiated mice (C576J/B1) to investigate the microenvironmental organization of marrow and identify early hematopoietic cell-stromal cell interactions. Undifferentiated colonies (UC) were detected at 3 days postirradiation, showed a marked predilection for bone surfaces, and disappeared as differentiated colonies developed. Some UC occurred along marrow arteries. Neutrophilic granulocyte colonies (GC) occurred in all areas at 3 days but grew rapidly only subosteally. Few eosinophilic colonies (GCe) occurred. Erythrocytic colonies (EC) appeared at 4 days as dispersed populations of motile cells within a localized area of marrow; these tended to proliferate initially in intermediate and central marrow zones. Macrophage colonies (M phi C) of two ''subtypes'' were detected, peaking in relative frequency at 4 days. These appeared active in stromal repair and monocytopoiesis. Megakaryocyte colonies (MC) originated along bone and differentiated away from bone. These results were interpreted as evidence that in x-irradiated marrow: (1) hematopoietic microenvironments (HMs) for stem-cell proliferation and commitment to differentiation, with the possible exception of HMs determining erythroid differentiation, occur in endosteal and periarterial regions; (2) a proliferative and/or chemotactic stimulus to erythroid progenitors exists in intermediate and central marrow regions; and (3) somemore » subosteal regions may exclude erythropoiesis, or preferentially support nonerythroid differentiation. Elaborate associations occurred between macrophages and early UC, GC, and EC, but not MC hematopoietic cells. UC and GC often associated with osteoclasts. Reticular and other fibroblastic cells associated with the cells of all colony types.« less

Authors:
;
Publication Date:
Research Org.:
Univ. of Pennsylvania, Philadelphia
OSTI Identifier:
5019356
Resource Type:
Journal Article
Journal Name:
Am. J. Anat.; (United States)
Additional Journal Information:
Journal Volume: 166:4
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BONE MARROW; BIOLOGICAL RADIATION EFFECTS; BONE MARROW CELLS; BLOOD FORMATION; CELL DIFFERENTIATION; CELL PROLIFERATION; COLONY FORMATION; MICE; X RADIATION; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; BIOLOGICAL EFFECTS; BODY; CONNECTIVE TISSUE CELLS; ELECTROMAGNETIC RADIATION; HEMATOPOIETIC SYSTEM; IONIZING RADIATIONS; MAMMALS; ORGANS; RADIATION EFFECTS; RADIATIONS; RODENTS; SOMATIC CELLS; TISSUES; VERTEBRATES; 560152* - Radiation Effects on Animals- Animals

Citation Formats

Lambertsen, R H, and Weiss, L. Studies on the organization and regeneration of bone marrow: origin, growth, and differentiation of endocloned hematopoietic colonies. United States: N. p., 1983. Web. doi:10.1002/aja.1001660402.
Lambertsen, R H, & Weiss, L. Studies on the organization and regeneration of bone marrow: origin, growth, and differentiation of endocloned hematopoietic colonies. United States. https://doi.org/10.1002/aja.1001660402
Lambertsen, R H, and Weiss, L. 1983. "Studies on the organization and regeneration of bone marrow: origin, growth, and differentiation of endocloned hematopoietic colonies". United States. https://doi.org/10.1002/aja.1001660402.
@article{osti_5019356,
title = {Studies on the organization and regeneration of bone marrow: origin, growth, and differentiation of endocloned hematopoietic colonies},
author = {Lambertsen, R H and Weiss, L},
abstractNote = {Hematopoietic colonies were studied by light microscopy in the marrow of alternate fraction x-irradiated mice (C576J/B1) to investigate the microenvironmental organization of marrow and identify early hematopoietic cell-stromal cell interactions. Undifferentiated colonies (UC) were detected at 3 days postirradiation, showed a marked predilection for bone surfaces, and disappeared as differentiated colonies developed. Some UC occurred along marrow arteries. Neutrophilic granulocyte colonies (GC) occurred in all areas at 3 days but grew rapidly only subosteally. Few eosinophilic colonies (GCe) occurred. Erythrocytic colonies (EC) appeared at 4 days as dispersed populations of motile cells within a localized area of marrow; these tended to proliferate initially in intermediate and central marrow zones. Macrophage colonies (M phi C) of two ''subtypes'' were detected, peaking in relative frequency at 4 days. These appeared active in stromal repair and monocytopoiesis. Megakaryocyte colonies (MC) originated along bone and differentiated away from bone. These results were interpreted as evidence that in x-irradiated marrow: (1) hematopoietic microenvironments (HMs) for stem-cell proliferation and commitment to differentiation, with the possible exception of HMs determining erythroid differentiation, occur in endosteal and periarterial regions; (2) a proliferative and/or chemotactic stimulus to erythroid progenitors exists in intermediate and central marrow regions; and (3) some subosteal regions may exclude erythropoiesis, or preferentially support nonerythroid differentiation. Elaborate associations occurred between macrophages and early UC, GC, and EC, but not MC hematopoietic cells. UC and GC often associated with osteoclasts. Reticular and other fibroblastic cells associated with the cells of all colony types.},
doi = {10.1002/aja.1001660402},
url = {https://www.osti.gov/biblio/5019356}, journal = {Am. J. Anat.; (United States)},
number = ,
volume = 166:4,
place = {United States},
year = {Fri Apr 01 00:00:00 EST 1983},
month = {Fri Apr 01 00:00:00 EST 1983}
}