Binding specificities of eight monoclonal antibodies to human glycophorin A - studies with M/sup c/M, and M/sub k/En(UK) variant human erythrocytes and M- and MN/sup V/-type chimpanzee erythrocytes

Journal Article · Sat Dec 01 00:00:00 EST 1984 · J. Immunol.; (United States)

OSTI ID:5019128

Bigbee, W L; Langlois, R G; Vanderlaan, M; Jensen, R H

Four newly derived mouse monoclonal antibodies to human glycophorin A are described. Three of these antibodies bind preferentially to the N form of glycophorin A; the fourth recognizes a shared determinant of the M and N forms. All four antibodies are directed toward the 39 amino acid, amino-terminal portion of the protein, and the N-specific antibodies require for binding the presence of N-acetyl-neuraminic acid on the glycosidically linked oligosaccharides. Cross-reaction of the N-specific antibodies to homozygous MM erythrocytes appears to result from binding to glycophorin B. In addition, these antibodies together with four previously reported glycophorin monoclonal antibodies, including two that specifically recognize the M form of glycophorin A, were tested for binding to M/sup c/M and M/sup k/En(UK) variant human erythrocytes. Results obtained for five of the six M- or N-specific monoclonal antibodies point to the general immunodominance of the amino-terminal serine-leucine polymorphism and the requirement for sialic acid. The epitopes for all three N-specific monoclonal antibodies include the amino terminal leucine that occurs in the N form of glycophorin A and may also include the glutamic acid that occurs at position five. Their studies support the proposed Lepore-type glycophorin A-B hybrid gene rearrangement for the En(UK) allele found in the English En(a-) family. The data also confirm the expression of the M-like glycoprotein on chimpanzee erythrocytes and the presence of a human glycophorin B-like antigen on the MN/sup V/-type cells.

• Research Organization: Lawrence Livermore National Lab., CA
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 5019128
• Journal Information: J. Immunol.; (United States), Vol. 133:6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOPROTEINS
ENZYME IMMUNOASSAY
MONOCLONAL ANTIBODIES
CELL FLOW SYSTEMS
ERYTHROCYTES
MAN
PRIMATES
ANIMALS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
IMMUNOASSAY
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
PROTEINS
SACCHARIDES
VERTEBRATES
550300* - Cytology