Developmental changes of beta-adrenergic receptor-linked adenylate cyclase of rat liver
beta-Adrenergic agonist-sensitive adenylate cyclase activity and binding of the beta-adrenergic antagonist(-)-(/sup 125/I)iodopindolol were studied in rat liver during development of male Fischer 344 rats ages 6-60 days. In liver homogenates maximum adenylate cyclase response to beta-adrenergic agonist (10(-5) M isoproterenol or epinephrine) decreased by 73% (P less than 0.01) between 6 and 60 days, with most of the decrease (56%; P less than 0.01) occurring by 20 days. beta-adrenergic receptor density (Bmax) showed a corresponding decrease of 66% (P less than 0.01) by 20 days without subsequent change. Binding characteristics of stereospecificity, pharmacological specificity, saturability with time, and reversibility were unchanged with age. GTP-, fluoride-, forskolin-, and Mn2+-stimulated adenylate cyclase activities also decreased during development, suggesting a decrease of activity of the catalytic component and/or guanine nucleotide regulatory component of adenylate cyclase. These results indicate that the developmental decrease of beta-adrenergic agonist-sensitive adenylate cyclase activity may result from decreased numbers of beta-adrenergic receptors. Developmental alterations of nonreceptor components of the enzyme may also contribute to changes of catecholamine-sensitive adenylate cyclase.
- OSTI ID:
- 5017276
- Journal Information:
- Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States)
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
CYCLASES
ENZYME ACTIVITY
FLUORIDES
BIOLOGICAL EFFECTS
GUANOSINE
MANGANESE COMPOUNDS
SYMPATHOMIMETICS
RECEPTORS
BIOCHEMICAL REACTION KINETICS
CATIONS
HOMOGENATES
IODINE 125
LABELLED COMPOUNDS
LIVER
NUCLEOTIDES
RATS
TRACER TECHNIQUES
ANIMALS
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AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
BETA DECAY RADIOISOTOPES
BODY
CHARGED PARTICLES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
FLUORINE COMPOUNDS
GLANDS
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
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ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LYASES
MAMMALS
NUCLEI
NUCLEOSIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PURINES
RADIOISOTOPES
REACTION KINETICS
RIBOSIDES
RODENTS
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES
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