Murine erythroleukemia cell line GM979 contains factors that can activate silent chromosomal human. gamma. -globin genes

Journal Article · Fri Mar 15 00:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.6.2530· OSTI ID:5015722

Zitnik, G; Hines, P; Stamatoyannopoulos, G; Papayannopoulou, T ^[1]

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1. Univ. of Washington, Seattle (United States)

The authors introduced a normal chromosome 11 into GM979 murine erythroleukemia cells by fusing them with Epstein-Barr virus-transformed lymphocytes from a normal individual. In contrast to precious data obtained with other murine erythroleukemia cells, they detected activation of human chromosomal {gamma}-globin genes in GM979 cells. GM979, unlike previously used murine erythroleukemia cell lines, expresses murine embryonic globin in addition to adult globin. While all the hybrids expressed {gamma}- and {beta}-globin, they displayed a wide range of {gamma}-globin expression in relation to that of {beta}-globin. No correlation, however, was found in quantitative expression between murine embryonic globin and human {gamma}-globin in these hybrids, suggesting that the two globins are regulated independently, at least in this cell line. These data indicate that {gamma}-globin genes from normal, nonerythroid chromosomes are not irreversibly silenced, and they can be activated by a positive trans factor(s) present in GM979 cells.

• OSTI ID: 5015722
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:6; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
CHEMICAL ACTIVATION
GLOBINS
GENE REGULATION
HETEROCHROMOSOMES
LEUKEMIA
LYMPHOCYTES
MAN
MESSENGER-RNA
MICE
PHOSPHORUS 32
TUMOR CELLS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
IMMUNE SYSTEM DISEASES
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
MAMMALS
MATERIALS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
RODENTS
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques