Mechanistic study on formaldehyde-induced hepatotoxicity

Journal Article · Sun Jan 01 00:00:00 EST 1989 · Journal of Toxicology and Environmental Health; (USA)

DOI:https://doi.org/10.1080/15287398909531306· OSTI ID:5015491

Strubelt, O; Younes, M; Pentz, R; Kuehnel, W ^[1]

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1. Medizinische Universitaet zu Luebeck (Germany, F.R.)

In isolated, hemoglobin-free perfused livers of fasted rats, formaldehyde at an initial concentration of 10 mmol/l produced toxicity as evidenced by a release of enzymes (GPT, SDH) and of glutathione (mainly GSSG) into the perfusate, an accumulation of calcium in the liver, and a depletion of hepatic glatathione. Formaldehyde also led to an enhanced release of malondialdehyde into the perfusate, indicating peroxidative processes and decreased hepatic oxygen consumption by about 50-70%. The electron microscopic investigation of formaldehyde-exposed livers showed a destruction of the mitochondria (ruptured membranes, loss of the cristae) and some damage of the rough endoplasmic reticulum. Feeding the rats prior to surgery attenuated the hepatotoxic effects of 10 mmol/l formaldehyde. At an initial concentration of 3 mmol/l, formaldehyde did not release enzymes from livers of fed or fasted rats but only from whose glutathione content had been depleted by treatment with phorone (250 mg/kg ip 2 h earlier). Formaldehyde liberated glucose and lactate from the livers of fed but not from those of fasted rats, indicating anaerobic energy supply in the fed state. The hepatotoxic action of formaldehyde is not due to its metabolism to formate or to the 10% methanol added as a stabilizing agent to the commercially available 37% solution named formalin.

• OSTI ID: 5015491
• Journal Information: Journal of Toxicology and Environmental Health; (USA), Vol. 27:3; ISSN 0098-4108
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
FORMALDEHYDE
TOXICITY
LIVER
PATHOLOGICAL CHANGES
ELECTRON MICROSCOPY
ENZYME ACTIVITY
LIVER CELLS
METABOLISM
RATS
ALDEHYDES
ANIMAL CELLS
ANIMALS
BODY
DIGESTIVE SYSTEM
GLANDS
MAMMALS
MICROSCOPY
ORGANIC COMPOUNDS
ORGANS
RODENTS
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology