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Title: Complete structure of the cell surface polysaccharide of Streptococcus oralis ATCC 10557: A receptor for lectin-mediated interbacterial adherence

Lectin-carbohydrate binding is known to play an important role in a number of different cell-cell interactions including those between certain species of oral streptococci and actinomyces that colonize teeth. The cell wall polysaccharides of Streptococcus oralis ATCC 10557, S. oralis 34, and Streptococcus mitis J22, although not identical antigenically, each function as a receptor molecule for the galactose and N-acetylgalactosamine reactive fimbrial lectins of Actinomyces viscosus and Actinomyces naeslundii. Carbohydrate analysis of the receptor polysaccharide isolated from S. oralis ATCC 10557 shows galactose (3 mol), glucose (1 mol), GalNAc (1 mol), and rhamnose (1 mol). {sup 1}H NMR spectra of the polysaccharide show that is partially O-acetylated. Analysis of the {sup 1}H NMR spectrum of the de-O-acetylated polysaccharide shows that it is composed of repeating subunits containing six monosaccharides and that the subunits are joined by a phosphodiester linkage. The {sup 1}H and {sup 13}C NMR spectra were completely assigned by two-dimensional homonuclear correlation methods and by {sup 1}H-detected heteronuclear multiple-quantum correlation ({sup 1}H({sup 13}C)HMQC). The complete {sup 1}H and {sup 13}C assignment of the native polysaccharide was carried out by the same techniques augmented by a {sup 13}C-coupled hybrid HMQC-COSY method, which is shown to be especially useful formore » carbohydrates in which strong coupling and overlapping peaks in the {sup 1}H spectrum pose difficulties.« less
Authors:
;  [1] ;  [2]
  1. (Univ. of Maryland, Baltimore (United States))
  2. (National Inst. of Dental Research, Bethesda, MD (United States))
Publication Date:
OSTI Identifier:
5013914
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemistry; (United States); Journal Volume: 30:26
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; CELL WALL; MOLECULAR STRUCTURE; POLYSACCHARIDES; NUCLEAR MAGNETIC RESONANCE; ACETYLATION; ACTINOMYCES; CARBON 13; CHEMICAL SHIFT; LECTINS; PROTONS; RECEPTORS; STREPTOCOCCUS; ACYLATION; BACTERIA; BARYONS; CARBOHYDRATES; CARBON ISOTOPES; CELL CONSTITUENTS; CHEMICAL REACTIONS; ELEMENTARY PARTICLES; EVEN-ODD NUCLEI; FERMIONS; HADRONS; ISOTOPES; LIGHT NUCLEI; MAGNETIC RESONANCE; MEMBRANE PROTEINS; MICROORGANISMS; NUCLEI; NUCLEONS; ORGANIC COMPOUNDS; PROTEINS; RESONANCE; SACCHARIDES; STABLE ISOTOPES 550601* -- Medicine-- Unsealed Radionuclides in Diagnostics