Evidence that the subunit structure of gonadotropin receptor is preserved during regression of rat corpus luteum
The level of hCG/LH receptor has been shown to undergo marked changes during the life span of rat corpus luteum. To evaluate whether these fluctuations are due to changes in the receptor subunit structure or receptor protein content, the /sup 125/I-hCG binding activity and the receptor subunit structure were determined during different time periods of pseudopregnancy. The maximum /sup 125/I-hCG binding activity was observed on day 7, after which it decreased by 20 and 45% on day 11 and day 14, respectively. The Scatchard analysis of /sup 125/I-hCG binding data showed that the decrease in binding activity was caused by a change in the number of binding sites rather than a change in the binding affinity. The LH/hCG receptor in ovarian membranes obtained on days 7, 11 and 14 were characterized by the method of affinity cross-linking. All four subunits of the LH/hCG receptor were detected in the ovarian membranes at all stages while the intensity decreased parallel to a decrease in hCG binding from day 7 to day 14.
- Research Organization:
- Univ. of Michigan Medical School, Ann Arbor
- OSTI ID:
- 5009119
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Vol. 137:1
- Country of Publication:
- United States
- Language:
- English
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GONADOTROPINS
BIOCHEMICAL REACTION KINETICS
RECEPTORS
EXPERIMENTAL DATA
IODINE 125
LABELLED COMPOUNDS
LH
OVARIES
RATS
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
DATA
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FEMALE GENITALS
GONADS
HORMONES
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
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KINETICS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
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RADIOISOTOPES
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques