Influence of chloramine T iodination on the biological and immunological activity or the molecular radius of the human growth hormone molecule

Journal Article · Wed Jan 01 00:00:00 EST 1986 · J. Immunoassay; (United States)

DOI:https://doi.org/10.1080/01971528608060461· OSTI ID:5006159

Bartolini, P; Ribela, M T

Potential alterations of the somatotropic activity of human growth hormone (hGH) resulting from Chloramine T labelling reaction, iodination up to 2.7 atoms/molecule and indirect radiation effects, have been studied. Three 2X2 factorial assays, performed in hypophysectomized rats, failed to reveal any significant difference (P greater than 0.05) in true growth promoting activity between hGH and (127-I)hGH, even after storing the latter with 125-I. Similar results were obtained applying a sensitive and precise gel filtration technique for Stokes Radius determination and radioimmunoassay.

• Research Organization: Cidade Universitaria, Sao Paulo, Brasil
• OSTI ID: 5006159
• Journal Information: J. Immunoassay; (United States), Vol. 3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
STH
CHEMICAL RADIATION EFFECTS
RADIOIMMUNOASSAY
BIOLOGICAL FUNCTIONS
CHLORAMINES
IODINE 125
LABELLED COMPOUNDS
RATS
AMINES
ANIMALS
BETA DECAY RADIOISOTOPES
CHEMISTRY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FUNCTIONS
HORMONES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RODENTS
TRACER TECHNIQUES
VERTEBRATES
560114* - Radiation Effects on Biochemicals- In Animals- (-1987)