Physico-chemical methods for the study of polycyclic aromatic hydrocarbon - DNA interactions. Progress report, October 1, 1985-September 30, 1986

Technical Report · Fri Oct 10 00:00:00 EDT 1986

OSTI ID:5000033

Geacintov, N E

Previous linear dichroism measurements suggested that the (-)BPDE-DNA adducts are characterized by considerable interactions between the pyrene residue and the DNA bases. Such a conformation is consistent with intercalation, or partial intercalation. With the pyrene residue thus protected from the solvent environment, one might expect a low degree of accessibility to acrylamide quencher molecules. Since the fluorescence of these (-)BPDE-DNA adducts is indeed insensitive to acrylamide, the quenching results reported here are consistent with such a conformation. The covalent adducts derived from the binding of (+)BPDE to DNA appear to be 70% accessible to acrylamide, suggesting that a majority of these adducts reside at external binding sites, or in a locally disordered region of the DNA double helix. Again, these conclusions derived from the fluorescence quenching data are consistent with our previous linear dichroism results. 3 refs., 3 figs.

• Research Organization: New York Univ., NY (USA). Dept. of Chemistry
• DOE Contract Number: AC02-78EV04959
• OSTI ID: 5000033
• Report Number(s): DOE/EV/04959-8; ON: DE87000957
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
DNA ADDUCTS
FLUORESCENCE SPECTROSCOPY
ACRYLAMIDE
BENZOPYRENE
DICHROISM
MOLECULAR MODELS
SYNCHROTRON RADIATION
ADDUCTS
AMIDES
AROMATICS
BREMSSTRAHLUNG
CONDENSED AROMATICS
ELECTROMAGNETIC RADIATION
EMISSION SPECTROSCOPY
HYDROCARBONS
MATHEMATICAL MODELS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIATIONS
SPECTROSCOPY
560300* - Chemicals Metabolism & Toxicology
550200 - Biochemistry