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Title: Physico-chemical methods for the study of polycyclic aromatic hydrocarbon - DNA interactions. Progress report, October 1, 1985-September 30, 1986

Previous linear dichroism measurements suggested that the (-)BPDE-DNA adducts are characterized by considerable interactions between the pyrene residue and the DNA bases. Such a conformation is consistent with intercalation, or partial intercalation. With the pyrene residue thus protected from the solvent environment, one might expect a low degree of accessibility to acrylamide quencher molecules. Since the fluorescence of these (-)BPDE-DNA adducts is indeed insensitive to acrylamide, the quenching results reported here are consistent with such a conformation. The covalent adducts derived from the binding of (+)BPDE to DNA appear to be 70% accessible to acrylamide, suggesting that a majority of these adducts reside at external binding sites, or in a locally disordered region of the DNA double helix. Again, these conclusions derived from the fluorescence quenching data are consistent with our previous linear dichroism results. 3 refs., 3 figs.
Authors:
Publication Date:
OSTI Identifier:
5000033
Report Number(s):
DOE/EV/04959-8
ON: DE87000957
DOE Contract Number:
AC02-78EV04959
Resource Type:
Technical Report
Research Org:
New York Univ., NY (USA). Dept. of Chemistry
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; DNA ADDUCTS; FLUORESCENCE SPECTROSCOPY; ACRYLAMIDE; BENZOPYRENE; DICHROISM; MOLECULAR MODELS; SYNCHROTRON RADIATION; ADDUCTS; AMIDES; AROMATICS; BREMSSTRAHLUNG; CONDENSED AROMATICS; ELECTROMAGNETIC RADIATION; EMISSION SPECTROSCOPY; HYDROCARBONS; MATHEMATICAL MODELS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; RADIATIONS; SPECTROSCOPY 560300* -- Chemicals Metabolism & Toxicology; 550200 -- Biochemistry