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Title: Organ-specific gene expression in maize: The P-wr allele. Final report, August 15, 1993--August 14, 1996

The ultimate aim of our work is to understand how a regulatory gene produces a specific pattern of gene expression during plant development. Our model is the P-wr gene of maize, which produces a distinctive pattern of pigmentation of maize floral organs. We are investigating this system using a combination of classical genetic and molecular approaches. Mechanisms of organ-specific gene expression are a subject of intense research interest, as it is the operation of these mechanisms during eukaryotic development which determine the characteristics of each organism Allele-specific expression has been characterized in only a few other plant genes. In maize, organ-specific pigmentation regulated by the R, B, and Pl genes is achieved by differential transcription of functionally conserved protein coding sequences. Our studies point to a strikingly different mechanism of organ-specific gene expression, involving post-transcriptional regulation of the regulatory P gene. The novel pigmentation pattern of the P-wr allele is associated with differences in the encoded protein. Furthermore, the P-wr gene itself is present as a unique tandemly amplified structure, which may affect its transcriptional regulation.
Authors:
Publication Date:
OSTI Identifier:
489694
Report Number(s):
DOE/ER/20124--T3
ON: DE97006552; TRN: 97:004033
DOE Contract Number:
FG02-93ER20124
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: [1997]
Research Org:
Iowa State Univ. of Science and Technology, Ames, IA (United States)
Sponsoring Org:
USDOE Office of Energy Research, Washington, DC (United States)
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; MAIZE; GENE REGULATION; TRANSCRIPTION; GENES; CLONING; DNA SEQUENCING; PROGRESS REPORT