Cometabolic degradation of chlorinated solvents: Bacterial inhibition, inactivation, and recovery
- Oregon State Univ., Corvallis, OR (United States). Dept. of Civil Engineering
This paper summarizes an approach for quantifying degradation kinetics and bacterial activity changes during cometabolic degradation of chlorinated solvents, results from trichloroethylene (TCE) degradation experiments, and a mathematical model addressing fluctuations in activity caused by enzyme inhibition, inactivation, and respondent enzyme synthesis. Using Nitrosomonas duropaea as a slow-growing exemplar capable of effecting cometabolic transformations, quasi-steady-state ammonia oxidation was established in a small bioreactor. A chlorinated solvent was injected to perturb the system, and bacterial activity and solvent degradation were monitored. At TCE concentrations to about 3.5 mg/L, from slight to nearly complete ammonia monooxygenase (AMO) inactivation occurred without causing immediate cell death. Results suggested cellular injury was limited primarily to AMO, most metabolic systems remained functional, and bacterial recovery processes, independent of cell growth, were initiated while degrading TCE.
- OSTI ID:
- 474276
- Report Number(s):
- CONF-950483-; ISBN 1-57477-005-5; TRN: IM9723%%166
- Resource Relation:
- Conference: 3. international in situ and on-site bioreclamation symposium, San Diego, CA (United States), 24-27 Apr 1995; Other Information: PBD: 1995; Related Information: Is Part Of Bioremediation of chlorinated solvents; Hinchee, R.E.; Leeson, A. [eds.] [Battelle Memorial Inst., Columbus, OH (United States)]; Semprini, L. [ed.] [Oregon State Univ., Corvallis, OR (United States)]; PB: 348 p.; Bioremediation, Volume 3(4)
- Country of Publication:
- United States
- Language:
- English
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