skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Mouse microsomal triglyceride transfer protein large subunit: cDNA cloning, tissue-specific expression, and chromosomal localization

Abstract

Microsomal triglyceride transfer protein (MTP) catalyzes the transfer of triglyceride, cholesteryl ester, and phospholipid between membranes. It is essential for the secretion of apolipoprotein B from the cell. Mutations in MTP are a major cause of abetalipoproteinemia. The mouse is a popular animal model for lipoprotein metabolism. We have cloned and sequenced mouse MTP cDNA. The DNA-deduced amino acid sequence indicates that mouse protein shows 93, 86, and 83% sequence indicates that mouse MTP contains 894 amino acids; the mouse protein shows 93, 86, and 83% sequence identity to the hamster, human, and bovine sequences, respectively. Northern blot analysis indicates that mouse MTP mRNA is expressed at high levels in the small intestine and at substantially lower levels in the liver and that it is not detectable in six other tissues examined. The mouse MTP gene has been localized to the distal region of chromosome 3 by Southern blots of interspecific backcross panels using progeny derived from matings of (C57BL/6J x SPRET/Ei)F1 x SPRET/Ei. Comparison of MTP sequences from human, bovine, hamster, and mouse indicates that the C-terminal region of MTP is better conserved than its N-terminal region. 21 refs., 2 figs.

Authors:
; ;  [1]
  1. Baylor College of Medicine, Houston, TX (United States); and others
Publication Date:
OSTI Identifier:
465965
Resource Type:
Journal Article
Journal Name:
Genomics
Additional Journal Information:
Journal Volume: 33; Journal Issue: 2; Other Information: PBD: 15 Apr 1996
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; PROTEINS; DNA-CLONING; DNA SEQUENCING; GENETIC MAPPING; GENE REGULATION; TISSUE DISTRIBUTION; GENE MUTATIONS; CHROMOSOMES; MICE; HYBRIDIZATION; HEREDITARY DISEASES; METABOLIC DISEASES; BIOLOGICAL MODELS; LIPOPROTEINS; METABOLISM; HUMAN CHROMOSOMES; AMINO ACID SEQUENCE; DNA HYBRIDIZATION; MICROSOMES; TRIGLYCERIDES; POLYMERASE CHAIN REACTION

Citation Formats

Nakamuta, Makoto, Chang, Benny Hung-Junn, and Hoogeveen, R. Mouse microsomal triglyceride transfer protein large subunit: cDNA cloning, tissue-specific expression, and chromosomal localization. United States: N. p., 1996. Web. doi:10.1006/geno.1996.0200.
Nakamuta, Makoto, Chang, Benny Hung-Junn, & Hoogeveen, R. Mouse microsomal triglyceride transfer protein large subunit: cDNA cloning, tissue-specific expression, and chromosomal localization. United States. https://doi.org/10.1006/geno.1996.0200
Nakamuta, Makoto, Chang, Benny Hung-Junn, and Hoogeveen, R. 1996. "Mouse microsomal triglyceride transfer protein large subunit: cDNA cloning, tissue-specific expression, and chromosomal localization". United States. https://doi.org/10.1006/geno.1996.0200.
@article{osti_465965,
title = {Mouse microsomal triglyceride transfer protein large subunit: cDNA cloning, tissue-specific expression, and chromosomal localization},
author = {Nakamuta, Makoto and Chang, Benny Hung-Junn and Hoogeveen, R},
abstractNote = {Microsomal triglyceride transfer protein (MTP) catalyzes the transfer of triglyceride, cholesteryl ester, and phospholipid between membranes. It is essential for the secretion of apolipoprotein B from the cell. Mutations in MTP are a major cause of abetalipoproteinemia. The mouse is a popular animal model for lipoprotein metabolism. We have cloned and sequenced mouse MTP cDNA. The DNA-deduced amino acid sequence indicates that mouse protein shows 93, 86, and 83% sequence indicates that mouse MTP contains 894 amino acids; the mouse protein shows 93, 86, and 83% sequence identity to the hamster, human, and bovine sequences, respectively. Northern blot analysis indicates that mouse MTP mRNA is expressed at high levels in the small intestine and at substantially lower levels in the liver and that it is not detectable in six other tissues examined. The mouse MTP gene has been localized to the distal region of chromosome 3 by Southern blots of interspecific backcross panels using progeny derived from matings of (C57BL/6J x SPRET/Ei)F1 x SPRET/Ei. Comparison of MTP sequences from human, bovine, hamster, and mouse indicates that the C-terminal region of MTP is better conserved than its N-terminal region. 21 refs., 2 figs.},
doi = {10.1006/geno.1996.0200},
url = {https://www.osti.gov/biblio/465965}, journal = {Genomics},
number = 2,
volume = 33,
place = {United States},
year = {Mon Apr 15 00:00:00 EDT 1996},
month = {Mon Apr 15 00:00:00 EDT 1996}
}