Action of a 50 Hz magnetic field on proliferation of cells in culture
- Forschungszentrum Karlsruhe (Germany). Institut fuer Toxikologie
Proliferation of SV40-3T3 mouse fibroblasts and human HL-60 promyelocytes was studied after treatment with a sinusoidal 2 mT{sub rms} 50 Hz magnetic field. A single exposure of 60 minutes caused quasicyclic changes in the cell number of SV40-3T3 cultures as function of time after treatment, which was interpreted to be due to the induction of chronobiological mechanisms by the field. Moreover, small variations in cell cycle distribution were measured during postexposure incubation for both cell lines. To discriminate between the effect of the magnetic vector and the induced electric field, HL-60 cell exposure was also performed on organ culture dishes. These dishes consist of two coaxially centered, isolated compartments in which different electric field levels are induced in the medium during treatment. Cell growth was affected in the outer compartment only where the induced electric field ranged from 8 to 12 mV{sub peak}/meter at 2 mT, but it was not affected in the inner compartment (field range 0--4 mV{sub peak}/meter). This suggests that the effects on cell growth are due to the induced electric field and are expressed only above a threshold of between 4 and 8 mV{sub peak}/meter.
- OSTI ID:
- 462587
- Journal Information:
- Bioelectromagnetics, Vol. 18, Issue 2; Other Information: PBD: 1997
- Country of Publication:
- United States
- Language:
- English
Similar Records
The induction of monocytopoiesis in HL-60 promyelocytic leukemia cells is inhibited by hydroquinone, a toxic metabolite of benzene
GROWTH REGULATION IN RSV INFECTED CHECKEN EMBRYO FIBROBLASTS: THE ROLE OF THE src GENE