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Title: Biomonitoring of aquatic sediments from Estonia with a fish liver cell line (PLHC-1)

Sediments were collected from lake Vorstjaerv and River Narva, Estonia, and extracted with dichloromethane. PLHC-1 cells were exposed to the extracts of the dry sediments. Cytotoxicity (total protein content) and CYP1A inducibility (EROD activity) was detected in the exposed cells. The contents of selected PAHs in the R. Narva sediments were also analyzed. The sediments collected from R. Narva were more toxic and caused higher EROD induction in the cells than the sediments from L. Vortsjaerv. The sediments collected from the northern part of the L. Vortsjaerv showed cytotoxicity. The sediments collected from the southern part of L. Vortsjaerv showed slight induction in EROD activity. In R. Narva, the most toxic sediments were collected downstream from the city of Narva and the Baltic Thermal Power Plant. At sublethal level of the sediment concentrations, also the highest EROD activities were seen at these downstream sites. At the sites heavily polluted with PAHs, also the EROD activities were high. The correlation between the PAH contents and EROD activities was not so evident when the sediments contained less PAHs. The PLHC-1 cells could serve as a sensitive method for screening cytotoxic and enzyme-inducing effects of the sediment extracts. This helps in evaluating themore » potential hazard of the compounds bound in the sediments for aquatic life. Further, the PLHC-1 cells can be used in comparing the properties of the sediments collected from different lakes and rivers.« less
Authors:
; ; ;  [1] ;
  1. Univ. of Kuopio (Finland). Dept. of Physiology
Publication Date:
OSTI Identifier:
452044
Report Number(s):
CONF-961149--
Journal ID: ISSN 1087-8939; TRN: IM9715%%76
Resource Type:
Conference
Resource Relation:
Conference: 17. annual meeting of the Society of Environmental Toxicology and Chemistry: partnerships for the environment - science, education, and policy, Washington, DC (United States), 17-21 Nov 1996; Other Information: PBD: 1995; Related Information: Is Part Of SETAC 17. annual meeting -- Abstract book. Partnerships for the environment: Science, education, and policy; PB: 378 p.
Publisher:
Society of Environmental Toxicology and Chemistry, Pensacola, FL (United States)
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; BIOLOGICAL INDICATORS; WATER POLLUTION; POLYCYCLIC AROMATIC HYDROCARBONS; ESTONIA; FISHES; MONITORING; OXIDOREDUCTASES; ENZYME ACTIVITY; BIOLOGICAL MARKERS