Sequential injection analysis of ethanol using immobilized alcohol dehydrogenase
Abstract
A Sequential Injection (SI) system was used to analyze the ethanol concentration in fermentation broth. The method is based on the use of immobilized NAD{sup +} dependent alcohol dehydrogenase. A non-linear standard curve for ethanol (range 0.25-100 mM) was used to determine the concentration in fermentation broth and the results correlated well with HPLC measurements. The assay time was 140 s, 0.5 {mu}mol of cofactor was used for each determination, and the relative standard deviation was less than 6% when analyzing fermentation samples. The assay system is very stable and makes it possible to reduce the cofactor consumption while keeping the system set up simple.
- Authors:
-
- Lund Univ. (Sweden)
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 433460
- Resource Type:
- Journal Article
- Journal Name:
- Analytical Letters
- Additional Journal Information:
- Journal Volume: 29; Journal Issue: 7; Other Information: PBD: May 1996
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 40 CHEMISTRY; 44 INSTRUMENTATION, INCLUDING NUCLEAR AND PARTICLE DETECTORS; ETHANOL; QUANTITATIVE CHEMICAL ANALYSIS; IMMOBILIZED ENZYMES; ALCOHOL DEHYDROGENASE; FERMENTATION; CALIBRATION; LIQUID COLUMN CHROMATOGRAPHY
Citation Formats
Hedenfalk, M, and Mattiasson, B. Sequential injection analysis of ethanol using immobilized alcohol dehydrogenase. United States: N. p., 1996.
Web. doi:10.1080/00032719608001462.
Hedenfalk, M, & Mattiasson, B. Sequential injection analysis of ethanol using immobilized alcohol dehydrogenase. United States. https://doi.org/10.1080/00032719608001462
Hedenfalk, M, and Mattiasson, B. 1996.
"Sequential injection analysis of ethanol using immobilized alcohol dehydrogenase". United States. https://doi.org/10.1080/00032719608001462.
@article{osti_433460,
title = {Sequential injection analysis of ethanol using immobilized alcohol dehydrogenase},
author = {Hedenfalk, M and Mattiasson, B},
abstractNote = {A Sequential Injection (SI) system was used to analyze the ethanol concentration in fermentation broth. The method is based on the use of immobilized NAD{sup +} dependent alcohol dehydrogenase. A non-linear standard curve for ethanol (range 0.25-100 mM) was used to determine the concentration in fermentation broth and the results correlated well with HPLC measurements. The assay time was 140 s, 0.5 {mu}mol of cofactor was used for each determination, and the relative standard deviation was less than 6% when analyzing fermentation samples. The assay system is very stable and makes it possible to reduce the cofactor consumption while keeping the system set up simple.},
doi = {10.1080/00032719608001462},
url = {https://www.osti.gov/biblio/433460},
journal = {Analytical Letters},
number = 7,
volume = 29,
place = {United States},
year = {Wed May 01 00:00:00 EDT 1996},
month = {Wed May 01 00:00:00 EDT 1996}
}
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