Molecular cloning and characterization of the mouse carboxyl ester lipase gene and evidence for expression in the lactating mammary gland
Abstract
DNA hybridization was used to isolate a 2.04-kb cDNA encoding carboxyl ester lipase (CEL) from a mouse lactating mammary gland, {lambda}gt10 cDNA library. The cDNA sequence translated into a protein of 599 amino acids, including 20 amino acids of a putative signal peptide. Comparison of the deduced amino acid sequence of the mouse CEL with CEL from five other species revealed that there is a high degree of a homology between the different species. The mouse CEL gene was also isolated and found to span approximately 7.2 kb and to include 11 exons. This organization is similar to those of the recently reported human and rat CEL genes. We have also analyzed expression of the CEL gene in the mammary glands from other species by performing a Northern blot analysis with RNA from goat and cow. The results show that the gene is expressed in both species. 36 refs., 6 figs., 1 tab.
- Authors:
-
- Astra Haessle AB, Moelndal (Sweden)
- Goeteborg Univ. (Sweden); and others
- Publication Date:
- OSTI Identifier:
- 433291
- Resource Type:
- Journal Article
- Journal Name:
- Genomics
- Additional Journal Information:
- Journal Volume: 29; Journal Issue: 1; Other Information: PBD: 1 Sep 1995
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; LIPASES; DNA SEQUENCING; DNA-CLONING; DNA HYBRIDIZATION; GENE REGULATION; AMINO ACID SEQUENCE; GENES; PEPTIDES; MICE; MAMMARY GLANDS
Citation Formats
Lidmer, A S, Lundberg, L, Kannius, M, and Bjursell, G. Molecular cloning and characterization of the mouse carboxyl ester lipase gene and evidence for expression in the lactating mammary gland. United States: N. p., 1995.
Web. doi:10.1006/geno.1995.1221.
Lidmer, A S, Lundberg, L, Kannius, M, & Bjursell, G. Molecular cloning and characterization of the mouse carboxyl ester lipase gene and evidence for expression in the lactating mammary gland. United States. https://doi.org/10.1006/geno.1995.1221
Lidmer, A S, Lundberg, L, Kannius, M, and Bjursell, G. 1995.
"Molecular cloning and characterization of the mouse carboxyl ester lipase gene and evidence for expression in the lactating mammary gland". United States. https://doi.org/10.1006/geno.1995.1221.
@article{osti_433291,
title = {Molecular cloning and characterization of the mouse carboxyl ester lipase gene and evidence for expression in the lactating mammary gland},
author = {Lidmer, A S and Lundberg, L and Kannius, M and Bjursell, G},
abstractNote = {DNA hybridization was used to isolate a 2.04-kb cDNA encoding carboxyl ester lipase (CEL) from a mouse lactating mammary gland, {lambda}gt10 cDNA library. The cDNA sequence translated into a protein of 599 amino acids, including 20 amino acids of a putative signal peptide. Comparison of the deduced amino acid sequence of the mouse CEL with CEL from five other species revealed that there is a high degree of a homology between the different species. The mouse CEL gene was also isolated and found to span approximately 7.2 kb and to include 11 exons. This organization is similar to those of the recently reported human and rat CEL genes. We have also analyzed expression of the CEL gene in the mammary glands from other species by performing a Northern blot analysis with RNA from goat and cow. The results show that the gene is expressed in both species. 36 refs., 6 figs., 1 tab.},
doi = {10.1006/geno.1995.1221},
url = {https://www.osti.gov/biblio/433291},
journal = {Genomics},
number = 1,
volume = 29,
place = {United States},
year = {Fri Sep 01 00:00:00 EDT 1995},
month = {Fri Sep 01 00:00:00 EDT 1995}
}