Genetic transformation of xylose-fermenting yeast Pichia stipitis
- Purdue Univ., West Lafayette, IN (United States)
A plasmid-mediated transformation system has been developed for the xylose-fermenting yeast Pichia stipitis. We found that plasmid vectors containing the Saccharomyces cerevisiae 2 p replicon and the kanamycin resistance gene (KmR) could be introduced into the Pichia cells and maintained as extrachromosomal elements. Pichia transformants containing such vectors will be resistant to the antibiotic geneticin that can be inactivated by the protein product of KmR. Plasmids identical to those used for transformation can be recovered from the Pichia transformants. Protocols for transformation of P. stipitis by the CaCl{sub 2}-polyethylene glycol-protoplast process or by direct electroporation of intact Pichia cells have both been developed.
- Research Organization:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Badger Engineers, Inc., Tampa, FL (United States); Solar Energy Research Inst., Golden, CO (United States)
- OSTI ID:
- 431595
- Report Number(s):
- CONF-900512-; TRN: 96:006510-0033
- Resource Relation:
- Conference: 12. symposium on biotechnology fuels and chemicals, Gatlinburg, TN (United States), 7-11 May 1990; Other Information: PBD: 1991; Related Information: Is Part Of Twelfth symposium on biotechnology for fuels and chemicals; Greenbaum, E. [ed.] [Oak Ridge National Lab., TN (United States)]; Wyman, C.E. [ed.] [Solar Energy Research Inst., Golden, CO (United States)]; PB: 934 p.
- Country of Publication:
- United States
- Language:
- English
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