Cellulase production from spent sulfite liquor and paper-mill waste fiber
Since a high proportion of the overall cost of the conversion of cellulosics to useful products is the expense of cellulose production (1), it is desirable to develop new processes for producing large amounts of cellulase inexpensively. So far, most of the research work on cellulose production has been carried out using milled cellulose powder and inorganic salts as substrates, which significantly increases the cost of enzyme production. In order to reduce the cost of raw materials, we tried to develop from industrial wastes a new medium for the production of cellulose. In this report, we describe a simple method by which an all-waste medium, which was composed of spent ammonium sulfite liquor and cellulosic waste of a paper mill, and a catabolite derepression mutant of Penicillium decumbens were used to produce the enzyme efficiently.
- Publication Date:
- OSTI Identifier:
- Report Number(s):
- Resource Type:
- Resource Relation:
- Conference: 12. symposium on biotechnology fuels and chemicals, Gatlinburg, TN (United States), 7-11 May 1990; Other Information: PBD: 1991; Related Information: Is Part Of Twelfth symposium on biotechnology for fuels and chemicals; Greenbaum, E. [ed.] [Oak Ridge National Lab., TN (United States)]; Wyman, C.E. [ed.] [Solar Energy Research Inst., Golden, CO (United States)]; PB: 934 p.
- Humana Press, Clifton, NJ (United States)
- Research Org:
- Oak Ridge National Lab., TN (United States); Badger Engineers, Inc., Tampa, FL (United States); Solar Energy Research Inst., Golden, CO (United States)
- Country of Publication:
- United States
- 32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; CELLULASE; BIOSYNTHESIS; CELLULOSE; CONVERSION; SPENT LIQUORS; BIOTECHNOLOGY; COST; INDUSTRIAL WASTES; PENICILLIUM; SUBSTRATES; PAPER INDUSTRY; WASTE MANAGEMENT