Induction and characterization of hepatic metallothionein in the channel catfish following exposure to cadmium
- Univ. of Arkansas, Little Rock, AR (United States)
The channel catfish (Ictalurus punctatus) is a commercially important fish found throughout the United States. Thus, understanding how this organism responds to environmental contaminants is of critical concern. The stress protein, metallothionein allows organisms to cope with excessive exposures of heavy metals, such as cadmium. Following a 5 mg/kg intraperitoneal injection of cadmium chloride, two metal binding proteins of approximately 8 kilodaltons and two MRNA bands of approximately 400 base pairs were induced in liver homogenates of juvenile channel catfish. Spectrophotometric examination of the purified proteins indicated that each protein was an isoform of metallothionein. Static aqueous exposures of 7 day duration to varied doses of cadmium chloride increased expression of metallothionein mRNA in a dose dependent manner. Significant (p {<=} 0.5) expression of MRNA was observed following exposures to cadmium chloride of 50/{mu}g/L or higher, but expression was unaltered by exposure to 10 {mu}g/L. Future studies will examine the time course of hepatic metallothionein expression in channel catfish following cadmium chloride exposure.
- OSTI ID:
- 40079
- Report Number(s):
- CONF-9410273-; TRN: IM9520%%274
- Resource Relation:
- Conference: 15. annual meeting of the Society of Environmental Toxicology and Chemistry (SETAC), Denver, CO (United States), 30 Oct - 3 Nov 1994; Other Information: PBD: 1994; Related Information: Is Part Of Society of Environmental Toxicology and Chemistry 15th annual meeting: Abstract book. Ecological risk: Science, policy, law, and perception; PB: 286 p.
- Country of Publication:
- United States
- Language:
- English
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