Stress protein expression in fish liver as a biomarker for environmental monitoring
- Environment Consultants, Vancouver, British Columbia (Canada)
Fish livers play a central role in xenobiotic metabolism and the induction of detoxifying enzymes such as cytochrome P450 in response to environmental pollutants has been well characterized in this organ. However, studies indicate that physiological changes, such as reproductive activity, and environmental variables, such as food availability, modify enzyme activities thereby limiting the use of hepatic enzymes as indicators of contaminant exposure. Stress proteins (SP) are a class of proteins induced by a wide variety of environmental stressors. A rainbow trout (Oncorhynchus mykiss) hepatocyte primary culture has been established to characterize and validate the use of SP expression as biomarkers of contaminant exposure. Hepatocytes were isolated by in situ perfusion of the liver with collagenase and the cells plated and kept at 15{degree}C. SDSPAGE and Western immunoblotting using antibodies raised against trout SP70 were used to determine the production of SP. Stress protein induction in response to heat shock and the toxicants cadmium chloride and {beta}-naphthoflavone have been characterized in the hepatocyte culture. Ongoing studies will describe the effects of bleached kraft pulp mill effluent on the hepatocytes. Modulation of those effects by the stress hormone cortisol is also being studied.
- OSTI ID:
- 392333
- Report Number(s):
- CONF-9511137-; ISBN 1-880611-03-1; TRN: IM9647%%187
- Resource Relation:
- Conference: 2. Society of Environmental Toxicology and Chemistry (SETAC) world conference, Vancouver (Canada), 5-9 Nov 1995; Other Information: PBD: 1995; Related Information: Is Part Of Second SETAC world congress (16. annual meeting): Abstract book. Global environmental protection: Science, politics, and common sense; PB: 378 p.
- Country of Publication:
- United States
- Language:
- English
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