skip to main content

Title: Induction of sister chromatid exchange in the presence of gadolinium-DTPA and its reduction by dimethyl sulfoxide

The authors investigate the frequency of sister chromatid exchange (SCE) after the addition of gadolinium (Gd)-DTPA to venous blood samples. Venous blood was obtained from nonsmokers. Samples were incubated with Gd-DTPA alone or in combination with mitomycin C, cytarabine, and dimethyl sulfoxide (DMSO), and then evaluated for SCEs. The frequency of SCE increased with the concentration of Gd-DTPA and as each chemotherapeutic agent was added. Sister chromatid exchange frequencies were lower when the blood was treated with a combination of Gd-DTPA and DMSO compared with Gd-DTPA alone. The increase in frequency of SCE seen after the addition of Gd-DTPA was decreased by the addition of DMSO, indicating the production of hydroxyl radicals. The effect likely is dissociation-related. 14 refs., 6 tabs.
Authors:
; ;  [1] ;  [2]
  1. Tokyo Medical and Dental Univ., Yokohama (Japan)
  2. Yokohama City Univ. (Japan)
Publication Date:
OSTI Identifier:
391300
Resource Type:
Journal Article
Resource Relation:
Journal Name: Investigative Radiology; Journal Volume: 31; Journal Issue: 5; Other Information: PBD: May 1996
Country of Publication:
United States
Language:
English
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; HUMAN CHROMOSOMES; SISTER CHROMATID EXCHANGES; MUTATION FREQUENCY; GADOLINIUM; GENETIC EFFECTS; DMSO; DTPA; DNA; GENETIC RADIATION EFFECTS; DNA REPAIR; MUTATIONS; CHEMOTHERAPY; HYDROXYL RADICALS; RADIOPROTECTIVE SUBSTANCES; LYMPHOCYTES