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Title: Chromosomal loci of 50 human keratinocyte cDNAs assigned by fluorescence in situ hybridization

Abstract

The chromosomal loci of expressed genes provide useful information for a candidate gene approach to the genes responsible for genetic diseases. A large set of randomly isolated cDNAs catalogued by partial sequencing can serve as a resource for accessing and isolating these disease genes. Using fluorescence in situ hybridization, we examined the chromosomal loci of 217 human keratinocyte-derived cDNAs, with independent novel sequence tags at the 3{prime} end region. Among them, we determined the loci of 50 cDNAs. Single-pass sequencing of these from the 5{prime} ends indicated that 39 cDNAs still can be produced for new genes. These cDNAs with identified chromosomal loci are powerful tools that can be used to help elucidate the genes responsible for hereditary skin disorders. 42 refs., 3 figs., 2 tabs.

Authors:
; ;  [1]
  1. Uyoto Prefectural Univ. of Medicine (Japan); and others
Publication Date:
OSTI Identifier:
390931
Resource Type:
Journal Article
Journal Name:
Genomics
Additional Journal Information:
Journal Volume: 28; Journal Issue: 2; Other Information: PBD: 20 Jul 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; HUMAN CHROMOSOME 6; GENETIC MAPPING; GENES; DNA SEQUENCING; GENE REGULATION; DNA-CLONING; HEREDITARY DISEASES; KERATIN; DNA HYBRIDIZATION; FLUORESCENCE; SKIN DISEASES

Citation Formats

Morishima, Yohich, Ariyama, Takeshi, and Yamanishi, Kiyofumi. Chromosomal loci of 50 human keratinocyte cDNAs assigned by fluorescence in situ hybridization. United States: N. p., 1995. Web. doi:10.1006/geno.1995.1141.
Morishima, Yohich, Ariyama, Takeshi, & Yamanishi, Kiyofumi. Chromosomal loci of 50 human keratinocyte cDNAs assigned by fluorescence in situ hybridization. United States. https://doi.org/10.1006/geno.1995.1141
Morishima, Yohich, Ariyama, Takeshi, and Yamanishi, Kiyofumi. 1995. "Chromosomal loci of 50 human keratinocyte cDNAs assigned by fluorescence in situ hybridization". United States. https://doi.org/10.1006/geno.1995.1141.
@article{osti_390931,
title = {Chromosomal loci of 50 human keratinocyte cDNAs assigned by fluorescence in situ hybridization},
author = {Morishima, Yohich and Ariyama, Takeshi and Yamanishi, Kiyofumi},
abstractNote = {The chromosomal loci of expressed genes provide useful information for a candidate gene approach to the genes responsible for genetic diseases. A large set of randomly isolated cDNAs catalogued by partial sequencing can serve as a resource for accessing and isolating these disease genes. Using fluorescence in situ hybridization, we examined the chromosomal loci of 217 human keratinocyte-derived cDNAs, with independent novel sequence tags at the 3{prime} end region. Among them, we determined the loci of 50 cDNAs. Single-pass sequencing of these from the 5{prime} ends indicated that 39 cDNAs still can be produced for new genes. These cDNAs with identified chromosomal loci are powerful tools that can be used to help elucidate the genes responsible for hereditary skin disorders. 42 refs., 3 figs., 2 tabs.},
doi = {10.1006/geno.1995.1141},
url = {https://www.osti.gov/biblio/390931}, journal = {Genomics},
number = 2,
volume = 28,
place = {United States},
year = {Thu Jul 20 00:00:00 EDT 1995},
month = {Thu Jul 20 00:00:00 EDT 1995}
}