Chromosomal loci of 50 human keratinocyte cDNAs assigned by fluorescence in situ hybridization
Abstract
The chromosomal loci of expressed genes provide useful information for a candidate gene approach to the genes responsible for genetic diseases. A large set of randomly isolated cDNAs catalogued by partial sequencing can serve as a resource for accessing and isolating these disease genes. Using fluorescence in situ hybridization, we examined the chromosomal loci of 217 human keratinocyte-derived cDNAs, with independent novel sequence tags at the 3{prime} end region. Among them, we determined the loci of 50 cDNAs. Single-pass sequencing of these from the 5{prime} ends indicated that 39 cDNAs still can be produced for new genes. These cDNAs with identified chromosomal loci are powerful tools that can be used to help elucidate the genes responsible for hereditary skin disorders. 42 refs., 3 figs., 2 tabs.
- Authors:
-
- Uyoto Prefectural Univ. of Medicine (Japan); and others
- Publication Date:
- OSTI Identifier:
- 390931
- Resource Type:
- Journal Article
- Journal Name:
- Genomics
- Additional Journal Information:
- Journal Volume: 28; Journal Issue: 2; Other Information: PBD: 20 Jul 1995
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; HUMAN CHROMOSOME 6; GENETIC MAPPING; GENES; DNA SEQUENCING; GENE REGULATION; DNA-CLONING; HEREDITARY DISEASES; KERATIN; DNA HYBRIDIZATION; FLUORESCENCE; SKIN DISEASES
Citation Formats
Morishima, Yohich, Ariyama, Takeshi, and Yamanishi, Kiyofumi. Chromosomal loci of 50 human keratinocyte cDNAs assigned by fluorescence in situ hybridization. United States: N. p., 1995.
Web. doi:10.1006/geno.1995.1141.
Morishima, Yohich, Ariyama, Takeshi, & Yamanishi, Kiyofumi. Chromosomal loci of 50 human keratinocyte cDNAs assigned by fluorescence in situ hybridization. United States. https://doi.org/10.1006/geno.1995.1141
Morishima, Yohich, Ariyama, Takeshi, and Yamanishi, Kiyofumi. 1995.
"Chromosomal loci of 50 human keratinocyte cDNAs assigned by fluorescence in situ hybridization". United States. https://doi.org/10.1006/geno.1995.1141.
@article{osti_390931,
title = {Chromosomal loci of 50 human keratinocyte cDNAs assigned by fluorescence in situ hybridization},
author = {Morishima, Yohich and Ariyama, Takeshi and Yamanishi, Kiyofumi},
abstractNote = {The chromosomal loci of expressed genes provide useful information for a candidate gene approach to the genes responsible for genetic diseases. A large set of randomly isolated cDNAs catalogued by partial sequencing can serve as a resource for accessing and isolating these disease genes. Using fluorescence in situ hybridization, we examined the chromosomal loci of 217 human keratinocyte-derived cDNAs, with independent novel sequence tags at the 3{prime} end region. Among them, we determined the loci of 50 cDNAs. Single-pass sequencing of these from the 5{prime} ends indicated that 39 cDNAs still can be produced for new genes. These cDNAs with identified chromosomal loci are powerful tools that can be used to help elucidate the genes responsible for hereditary skin disorders. 42 refs., 3 figs., 2 tabs.},
doi = {10.1006/geno.1995.1141},
url = {https://www.osti.gov/biblio/390931},
journal = {Genomics},
number = 2,
volume = 28,
place = {United States},
year = {Thu Jul 20 00:00:00 EDT 1995},
month = {Thu Jul 20 00:00:00 EDT 1995}
}