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Title: Structure and chromosomal localization of the human gene of the phosphotyrosyl phosphatase activator (PTPA) of protein phosphatase 2A

Abstract

The PTPA gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. PTPA, cloned from human genomic libraries, is encoded by one single-copy gene, composed of 10 exons and 9 introns with a total length of about 60 kb. The transcription start site was determined, and the 5{prime} flanking sequence was analyzed for its potential as a promotor. This region lacks a TATA sequence in the appropriate position relative to the transcription start, is very GC-rich, and contains upstream of the transcription start four Sp1 sites, a feature common to many TATA-less promotors. Based on the homology with DNA binding consensus sequences of transcription factors, we identified in this promotor region several putative DNA binding sites for transcription factors, such as NF-{kappa}B, Myb, Ets-1, Myc, and ATF. Transfection experiments with a construct containing the PTPA promotor region inserted 5{prime} of a luciferase reporter gene revealed that the 5{prime} flanking sequence of the PTPA gene indeed displayed promotor activity that seems to be cell-line dependent. By fluorescence in situ hybridization and G-banding, the PTPA gene was localized to the 9q34 region. The PTPA gene is positioned centromeric of c-abl in a region embracing several genes implicatedmore » in oncogenesis. 28 refs., 8 figs., 1 tab.« less

Authors:
; ; ;  [1]
  1. Katholieke Universiteit Leuven, Parish (France); and others
Publication Date:
OSTI Identifier:
390930
Resource Type:
Journal Article
Journal Name:
Genomics
Additional Journal Information:
Journal Volume: 28; Journal Issue: 2; Other Information: PBD: 20 Jul 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; HUMAN CHROMOSOME 9; GENETIC MAPPING; GENES; DNA-CLONING; DNA SEQUENCING; TRANSCRIPTION; STRUCTURE-ACTIVITY RELATIONSHIPS; PHOSPHATASES; PHOSPHORYLATION; ONCOGENIC TRANSFORMATIONS; TRANSCRIPTION FACTORS; DNA HYBRIDIZATION; FLUORESCENCE; BANDING TECHNIQUES; RFLPS; POLYMERASE CHAIN REACTION; OLIGONUCLEOTIDES; AMINO ACID SEQUENCE

Citation Formats

Van Hoof, C, Cayla, X, Merlevede, W, and Goris, J. Structure and chromosomal localization of the human gene of the phosphotyrosyl phosphatase activator (PTPA) of protein phosphatase 2A. United States: N. p., 1995. Web. doi:10.1006/geno.1995.1140.
Van Hoof, C, Cayla, X, Merlevede, W, & Goris, J. Structure and chromosomal localization of the human gene of the phosphotyrosyl phosphatase activator (PTPA) of protein phosphatase 2A. United States. https://doi.org/10.1006/geno.1995.1140
Van Hoof, C, Cayla, X, Merlevede, W, and Goris, J. 1995. "Structure and chromosomal localization of the human gene of the phosphotyrosyl phosphatase activator (PTPA) of protein phosphatase 2A". United States. https://doi.org/10.1006/geno.1995.1140.
@article{osti_390930,
title = {Structure and chromosomal localization of the human gene of the phosphotyrosyl phosphatase activator (PTPA) of protein phosphatase 2A},
author = {Van Hoof, C and Cayla, X and Merlevede, W and Goris, J},
abstractNote = {The PTPA gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. PTPA, cloned from human genomic libraries, is encoded by one single-copy gene, composed of 10 exons and 9 introns with a total length of about 60 kb. The transcription start site was determined, and the 5{prime} flanking sequence was analyzed for its potential as a promotor. This region lacks a TATA sequence in the appropriate position relative to the transcription start, is very GC-rich, and contains upstream of the transcription start four Sp1 sites, a feature common to many TATA-less promotors. Based on the homology with DNA binding consensus sequences of transcription factors, we identified in this promotor region several putative DNA binding sites for transcription factors, such as NF-{kappa}B, Myb, Ets-1, Myc, and ATF. Transfection experiments with a construct containing the PTPA promotor region inserted 5{prime} of a luciferase reporter gene revealed that the 5{prime} flanking sequence of the PTPA gene indeed displayed promotor activity that seems to be cell-line dependent. By fluorescence in situ hybridization and G-banding, the PTPA gene was localized to the 9q34 region. The PTPA gene is positioned centromeric of c-abl in a region embracing several genes implicated in oncogenesis. 28 refs., 8 figs., 1 tab.},
doi = {10.1006/geno.1995.1140},
url = {https://www.osti.gov/biblio/390930}, journal = {Genomics},
number = 2,
volume = 28,
place = {United States},
year = {Thu Jul 20 00:00:00 EDT 1995},
month = {Thu Jul 20 00:00:00 EDT 1995}
}