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Title: Ultrasensitive nucleic acid sequence detection by single-molecule electrophoresis

This is the final report of a one-year laboratory-directed research and development project at Los Alamos National Laboratory. There has been considerable interest in the development of very sensitive clinical diagnostic techniques over the last few years. Many pathogenic agents are often present in extremely small concentrations in clinical samples, especially at the initial stages of infection, making their detection very difficult. This project sought to develop a new technique for the detection and accurate quantification of specific bacterial and viral nucleic acid sequences in clinical samples. The scheme involved the use of novel hybridization probes for the detection of nucleic acids combined with our recently developed technique of single-molecule electrophoresis. This project is directly relevant to the DOE`s Defense Programs strategic directions in the area of biological warfare counter-proliferation.
Authors:
;
Publication Date:
OSTI Identifier:
374265
Report Number(s):
LA-UR--96-3052
ON: DE96014721
DOE Contract Number:
W-7405-ENG-36
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: [1996]
Research Org:
Los Alamos National Lab., NM (United States)
Sponsoring Org:
USDOE, Washington, DC (United States)
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; 45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE; NUCLEIC ACIDS; DETECTION; BACTERIAL DISEASES; VIRAL DISEASES; PROGRESS REPORT; ELECTROPHORESIS; MOLECULAR BIOLOGY; PATHOGENS; QUANTITATIVE CHEMICAL ANALYSIS; BIOLOGICAL WARFARE