A test for evaluating the toxicity of oils, dispersants, and oil biodegradation products to embryos of grass shrimp (Palaemonetes pugio)
- Environmental Protection Agency, Gulf Breeze, FL (United States). Environmental Research Lab.
A test has been developed to determine the toxicity of oil, commercial oil dispersants, and oil biodegradation products to embryos of the grass shrimp, Palaemonetes pugio. The system has several advantages over traditional toxicity tests: Embryos are exposed separately in glass tubes eliminating interaction between individuals, they do not require feeding, are easily examined, and require low volumes of test toxicant. Additionally, tests can be performed using artificial sea salts and adult P. pugio can be maintained and cultured year round in the laboratory. Toxicity tests demonstrated that oil dispersants increased the toxicity of the water-soluble fraction of No. 2 Fuel Oil with estimated LC50 values approximating those obtained with the 7d chronic tests using Mysidopsis bahia. Embryos exposed to neutral fractions derived by microbial degradation of weathered Prudhoe Bay crude oil showed toxicity of metabolic products at relatively low concentrations and cause virtually 100% mortality within a narrow time interval. P. pugio embryos were especially responsive to oil metabolites, exhibiting high sensitivity and low variability. All tests showed a consistently high survival (90--100%) of control embryos to hatch under a variety of temperatures and salinities; embryos at any given test condition usually hatched within 24h of one another.
- OSTI ID:
- 37385
- Report Number(s):
- CONF-9410273-; TRN: IM9519%%309
- Resource Relation:
- Conference: 15. annual meeting of the Society of Environmental Toxicology and Chemistry (SETAC), Denver, CO (United States), 30 Oct - 3 Nov 1994; Other Information: PBD: 1994; Related Information: Is Part Of Society of Environmental Toxicology and Chemistry 15th annual meeting: Abstract book. Ecological risk: Science, policy, law, and perception; PB: 286 p.
- Country of Publication:
- United States
- Language:
- English
Similar Records
Comparison of toxicity test methods using embryos of the grass shrimp, Palaemonetes pugio
Developmental toxicity of copper chloride, methylene chloride, and 6-aminonicotinamide to embryos of the grass shrimp Palaemonetes pugio