Analysis of hexazinone in soil by enzyme linked immunosorbent assay
- Univ. of Maine, Orono, ME (United States); and others
A tube enzyme immunoassay (ELA) procedure was developed for the determination of the triazine herbicide hexazinone in soil. The antibody was polyclonal and was prepared by employing metabolite A (3-(4-hydroxycyclohexyl)-6-dimethylamino)-1-methyl-1,3,5-triazine-2,4(1H,3H)-dione of hexazinone conjugated to bovine serum albumin as the immunogen. Hexazinone was extracted from soil by shaking with methanol-water 80/20 for 10 min and allowed to set overnight before reshaking for 5 min. Aliquots for EIA analysis were diluted in such a way as to always contain 8% methanol. Reproducibility results for both standards and samples were good. A correlation coefficient of 0.9562 was obtained for 76 soil samples run by EIA vs. HPLC. Of the eight known metabolites of hexazinone, 7 were tested for cross-reactivity and 5 were shown to be cross-reactive.
- OSTI ID:
- 370227
- Report Number(s):
- CONF-960376-; TRN: 96:003805-0278
- Resource Relation:
- Conference: Spring national meeting of the American Chemical Society (ACS), New Orleans, LA (United States), 24-28 Mar 1996; Other Information: PBD: 1996; Related Information: Is Part Of 211th ACS national meeting; PB: 2284 p.
- Country of Publication:
- United States
- Language:
- English
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