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Title: Determination of transfer rate and nature of the residue(s) in milk from {sup 14}C-atrazine cows

In order to determine the rate of transfer and the nature of the atrazine residues present in milk, lactating dairy cattle were treated with atrazine at three concentrations, 0.764 ppm, 0.0747 ppm and 0.0085 ppm (dry weight of food consumed). The concentrations were selected to bridge the gap between the concentration used for EPA metabolism studies (10 ppm) and the potential exposure level of dairy cattle to atrazine and its chlorotriazine metabolites through feed. The cattle were dosed following the morning milking for nine consecutive days with a single capsule bolus of {sup 14}C-atrazine. Milk was collected twice daily and aliquots of each milking and the individual cow`s daily pool of milk were analyzed by liquid scinitllation counting (LSC). The concentrations of {sup 14}C-residues in the milk plateaued on approximately day 3 and the mean {sup 14}C-atrazine levels in milk were 11.2 ppb, 1.13 ppb and 0.152 ppb for the high, middle and low dosed animals, respectively. The transfer of radioactive level of exposure to {sup 14}C-atrazine. The nature of the residues in milk were determined by extracting milk samples and analysis by HPLC, TLC or Aminex chromatography. Diaminchlorotriazine was the only chlorinated metabolite in the milk, constituting approximately 65%more » to 75% of the total radioactive residues (TRR).« less
Authors:
;  [1] ;  [2]
  1. Corning Hazleton, Inc., Madison, WI (United States)
  2. Ciba-Geigy Corp., Greensboro, NC (United States)
Publication Date:
OSTI Identifier:
369969
Report Number(s):
CONF-960376--
TRN: 96:003805-0016
Resource Type:
Conference
Resource Relation:
Conference: Spring national meeting of the American Chemical Society (ACS), New Orleans, LA (United States), 24-28 Mar 1996; Other Information: PBD: 1996; Related Information: Is Part Of 211th ACS national meeting; PB: 2284 p.
Publisher:
American Chemical Society, Washington, DC (United States)
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; MILK; RADIOACTIVITY; ORGANIC CHLORINE COMPOUNDS; METABOLISM; TRACER TECHNIQUES; LABELLED COMPOUNDS; CARBON 14; CATTLE; METABOLITES; RESIDUES