Bioremediation of contaminated harbor sediments. 2: Monitoring of contaminant degradation

Vanderhaegen, B; De Brabandere, J; Dumon, G; Verstraete, W

Title: Bioremediation of contaminated harbor sediments. 2: Monitoring of contaminant degradation

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Abstract

A 3 phase project involving bench, pilot and full scale application aimed at bioremediation of polycyclic aromatic hydrocarbons (PAH), mineral oil and tributyltin (TBT) contaminated sediments. On bench scale, biodegradation of PAH was monitored in 1-liter aerobic completely mixed reactors. The effect of adding microbial associations, surfactants and nutrients was monitored. Three parallel tests were set up: one monitored by means of {sup 14}C-labelled naphthalene and benzo(a)pyrene, the other by GC-MSD analyses and a third by respirometry and HPLC. A shift from dichloromethane extractable {sup 14}C to non-extractable {sup 14}C for the labelled molecules was observed. Further analyses revealed that the nonextractable {sup 14}C was associated with the humines. The amount of {sup 14}C recovered as {sup 14}C-CO{sub 2} was 10% for benzo(a)pyrene and 54% for naphthalene. The results were not influenced by adding microbial inocula. Results of GC-MSD-measurements indicated an overall removal efficiency of 70% after 7 weeks (half-life: 1 month). Supplementary addition of bacteria sometimes gave slightly better results. During the pilot scale test, a systematic difference between GC-MSD and HPLC was detected. GC-MSD gave a bigger response on low molecular weight PAH. HPLC revealed higher concentrations for more complex PAH. Overall, GC-MSD was evaluated as the mostmore »« less

Authors:: Vanderhaegen, B; De Brabandere, J; Dumon, G; Verstraete, W

Publication Date:: Sun Dec 31 00:00:00 EST 1995

OSTI Identifier:: 367479

Report Number(s):: CONF-9511137-
ISBN 1-880611-03-1; TRN: IM9640%%225

Resource Type:: Conference

Resource Relation:: Conference: 2. Society of Environmental Toxicology and Chemistry (SETAC) world conference, Vancouver (Canada), 5-9 Nov 1995; Other Information: PBD: 1995; Related Information: Is Part Of Second SETAC world congress (16. annual meeting): Abstract book. Global environmental protection: Science, politics, and common sense; PB: 378 p.

Country of Publication:: United States

Language:: English

Subject:: 54 ENVIRONMENTAL SCIENCES; BELGIUM; REMEDIAL ACTION; HARBORS; SEDIMENTS; NAPHTHALENE; BIODEGRADATION; BENZOPYRENE; SITE CHARACTERIZATION; WATER POLLUTION; CARBON 14 COMPOUNDS; TRACER TECHNIQUES

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                    Vanderhaegen, B, De Brabandere, J, Dumon, G, and Verstraete, W. Bioremediation of contaminated harbor sediments. 2: Monitoring of contaminant degradation.  United States: N. p., 1995. 
        Web.

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                    Vanderhaegen, B, De Brabandere, J, Dumon, G, & Verstraete, W. Bioremediation of contaminated harbor sediments. 2: Monitoring of contaminant degradation.  United States.

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                    Vanderhaegen, B, De Brabandere, J, Dumon, G, and Verstraete, W. 1995.  
        "Bioremediation of contaminated harbor sediments. 2: Monitoring of contaminant degradation".  United States.

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@article{osti_367479,

  title        = {Bioremediation of contaminated harbor sediments. 2: Monitoring of contaminant degradation},

  author       = {Vanderhaegen, B and De Brabandere, J and Dumon, G and Verstraete, W},

  abstractNote = {A 3 phase project involving bench, pilot and full scale application aimed at bioremediation of polycyclic aromatic hydrocarbons (PAH), mineral oil and tributyltin (TBT) contaminated sediments. On bench scale, biodegradation of PAH was monitored in 1-liter aerobic completely mixed reactors. The effect of adding microbial associations, surfactants and nutrients was monitored. Three parallel tests were set up: one monitored by means of {sup 14}C-labelled naphthalene and benzo(a)pyrene, the other by GC-MSD analyses and a third by respirometry and HPLC. A shift from dichloromethane extractable {sup 14}C to non-extractable {sup 14}C for the labelled molecules was observed. Further analyses revealed that the nonextractable {sup 14}C was associated with the humines. The amount of {sup 14}C recovered as {sup 14}C-CO{sub 2} was 10% for benzo(a)pyrene and 54% for naphthalene. The results were not influenced by adding microbial inocula. Results of GC-MSD-measurements indicated an overall removal efficiency of 70% after 7 weeks (half-life: 1 month). Supplementary addition of bacteria sometimes gave slightly better results. During the pilot scale test, a systematic difference between GC-MSD and HPLC was detected. GC-MSD gave a bigger response on low molecular weight PAH. HPLC revealed higher concentrations for more complex PAH. Overall, GC-MSD was evaluated as the most valuable method. The experiment confirmed the bench scale kinetics. After 5 weeks the PAH-concentration remained constant. The overall removal efficiency was 70 to 90% after 5 months. Tributyltin and mineral oil were mineralized with about 70% (half-life: 15 weeks) and 90% respectively.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/367479},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Sun Dec 31 00:00:00 EST 1995},

  month        = {Sun Dec 31 00:00:00 EST 1995}

}

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