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Title: Monooxygenase-mediated 1,2-dichloroethane degradation by Pseudomonas sp. strain DCA1

Abstract

A bacterial strain, designated Pseudomonas sp. strain DCA1, was isolated from a 1,2-dichloroethane (DCA)-degrading biofilm. Strain DCA1 utilizes DCA as the sole carbon and energy source and does not require additional organic nutrients, such as vitamins, for optimal growth. The affinity of strain DCA1 for DCA is very high, with a K{sub m} value below the detection limit of 0.5 {micro}M. Instead of a hydrolytic dehalogenation, as in other DCA utilizers, the first step in DCA degradation in strain DCA1 is an oxidation reaction. Oxygen and NAD(P)H are required for this initial step. Propene was converted to 1,2-epoxypropane by DCA-grown cells and competitively inhibited DCA degradation. The authors concluded that a monooxygenase is responsible for the first step in DCA degradation in strain DCA1. Oxidation of DCA probably results in the formation of the unstable intermediate 1,2-dichloroethanol, which spontaneously releases chloride, yielding chloroacetaldehyde. The DCA degradation pathway is strain DCA1 proceeds from chloroacetaldehyde via chloroacetic acid and presumably glycolic acid, which is similar to degradation routes observed in other DCA-utilizing bacteria.

Authors:
;  [1]
  1. Wageningen Univ. (Netherlands)
Publication Date:
OSTI Identifier:
354326
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 65; Journal Issue: 6; Other Information: PBD: Jun 1999
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; REMEDIAL ACTION; BIODEGRADATION; CHLORINATED ALIPHATIC HYDROCARBONS; GROUND WATER; SOILS; OXYGENASES; PSEUDOMONAS

Citation Formats

Hage, J C, and Hartmans, S. Monooxygenase-mediated 1,2-dichloroethane degradation by Pseudomonas sp. strain DCA1. United States: N. p., 1999. Web.
Hage, J C, & Hartmans, S. Monooxygenase-mediated 1,2-dichloroethane degradation by Pseudomonas sp. strain DCA1. United States.
Hage, J C, and Hartmans, S. 1999. "Monooxygenase-mediated 1,2-dichloroethane degradation by Pseudomonas sp. strain DCA1". United States.
@article{osti_354326,
title = {Monooxygenase-mediated 1,2-dichloroethane degradation by Pseudomonas sp. strain DCA1},
author = {Hage, J C and Hartmans, S},
abstractNote = {A bacterial strain, designated Pseudomonas sp. strain DCA1, was isolated from a 1,2-dichloroethane (DCA)-degrading biofilm. Strain DCA1 utilizes DCA as the sole carbon and energy source and does not require additional organic nutrients, such as vitamins, for optimal growth. The affinity of strain DCA1 for DCA is very high, with a K{sub m} value below the detection limit of 0.5 {micro}M. Instead of a hydrolytic dehalogenation, as in other DCA utilizers, the first step in DCA degradation in strain DCA1 is an oxidation reaction. Oxygen and NAD(P)H are required for this initial step. Propene was converted to 1,2-epoxypropane by DCA-grown cells and competitively inhibited DCA degradation. The authors concluded that a monooxygenase is responsible for the first step in DCA degradation in strain DCA1. Oxidation of DCA probably results in the formation of the unstable intermediate 1,2-dichloroethanol, which spontaneously releases chloride, yielding chloroacetaldehyde. The DCA degradation pathway is strain DCA1 proceeds from chloroacetaldehyde via chloroacetic acid and presumably glycolic acid, which is similar to degradation routes observed in other DCA-utilizing bacteria.},
doi = {},
url = {https://www.osti.gov/biblio/354326}, journal = {Applied and Environmental Microbiology},
number = 6,
volume = 65,
place = {United States},
year = {Tue Jun 01 00:00:00 EDT 1999},
month = {Tue Jun 01 00:00:00 EDT 1999}
}