cDNA cloning, molecular characterization, and chromosomal localization of NET(EPHT2), a human EPH-related receptor protein-tyrosine kinase gene preferentially expressed in brain
- Children`s Hospital of Philadelphia, PA (United States); and others
By screening a human fetal brain cDNA expression library using a monoclonal anti-phosphotyrosine antibody , we have isolated a cDNA clone encoding a receptor type protein-tyrosine kinase belonging to the EPH family, NET (neuronally expressed EPH-related tyrosine kinase). NET shows 87% homology in nucleotide sequence and 99% homology in the deduced amino acid sequence to rat elk, suggesting that NET is the human homologue of elk. The NET gene is mapped to human chromosome 3q21-q23 by PCR screening of a human rodent somatic cell hybrid panel and by fluorescence in situ hybridization. Examination of NET mRNA expression in several human tissues has shown that the NET gene is expressed preferentially in brain as a 5-kb transcript. Steady-state levels of NET mRNA in human brain are greater in the midterm fetus than in the adult. Lower levels of NET mRNA are found in fetal kidney and adult skeletal muscle. The expression pattern of NET mRNA thus differs from that of elk, suggesting that these two gene products may preform distinct roles in human and rat. NET transcripts are detected in human acid-induced neuronal differentiation. Several human tumor cell lines derived from neuroectoderm including primitive neuroblastoma also express NET transcripts. Since the NET mRNA expression in human brain is developmentally regulated and is induced during neuronal differentiation, NET potentially plays important roles in human neurogenesis. 89 refs., 7 figs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 258281
- Journal Information:
- Genomics, Vol. 29, Issue 2; Other Information: PBD: 20 Sep 1995
- Country of Publication:
- United States
- Language:
- English
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BASIC STUDIES
HUMAN CHROMOSOME 3
GENETIC MAPPING
SOMATIC CELLS
HYBRIDIZATION
RECEPTORS
GENES
DNA-CLONING
TRANSCRIPTION
TISSUE DISTRIBUTION
GENE REGULATION
DNA SEQUENCING
PHOSPHOTRANSFERASES
NUCLEOTIDES
AMINO ACID SEQUENCE
RATS
POLYMERASE CHAIN REACTION
DNA HYBRIDIZATION
FLUORESCENCE
PROTEINS
TYROSINE
BRAIN
MESSENGER-RNA