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Title: Localization of multiple human dihydrodiol dehydrogenase (DDH1 and DDH2) and chlordecone reductase (CHDR) genes in chromosome 10 by the polymerase chain reaction and fluorescence in situ hybridization

Abstract

Multiple human dihydrodiol dehydrogenases and human chlordecone reductase belong to the aldo-keto reductase superfamily. These two enzymes are involved in the metabolism of xenobiotics, such as polycyclic aromatic hydrocarbons and pesticides. Recently we have isolated three closely related genes encoding two dihydrodiol dehydrogenases (DDH1 and DDH2) and the chlordecone reductase (CHDR). Mapping of the location of the genes was performed using the polymerase chain reaction using gene-specific primers to amplify gene sequences in human/hamster hybrid DNA. All three genes were found to be located on chromosome 10. In situ hybridization using a lambda clone as the probe further confirmed regional localization at 10p14-p15. 13 refs., 2 figs.

Authors:
; ;  [1]
  1. Cornell Univ. Medical College, New York, NY (United States); and others
Publication Date:
OSTI Identifier:
250177
Resource Type:
Journal Article
Journal Name:
Genomics
Additional Journal Information:
Journal Volume: 25; Journal Issue: 2; Other Information: PBD: 20 Jan 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; GENES; DNA-CLONING; DNA SEQUENCING; GENETIC MAPPING; HUMAN CHROMOSOMES; OXIDOREDUCTASES; XENOBIOTICS; METABOLISM; DNA HYBRIDIZATION; FLUORESCENCE; POLYMERASE CHAIN REACTION; PROBES; SOMATIC CELLS

Citation Formats

Khanna, M, Qin, K N, and Belkin, S. Localization of multiple human dihydrodiol dehydrogenase (DDH1 and DDH2) and chlordecone reductase (CHDR) genes in chromosome 10 by the polymerase chain reaction and fluorescence in situ hybridization. United States: N. p., 1995. Web. doi:10.1016/0888-7543(95)80066-U.
Khanna, M, Qin, K N, & Belkin, S. Localization of multiple human dihydrodiol dehydrogenase (DDH1 and DDH2) and chlordecone reductase (CHDR) genes in chromosome 10 by the polymerase chain reaction and fluorescence in situ hybridization. United States. https://doi.org/10.1016/0888-7543(95)80066-U
Khanna, M, Qin, K N, and Belkin, S. 1995. "Localization of multiple human dihydrodiol dehydrogenase (DDH1 and DDH2) and chlordecone reductase (CHDR) genes in chromosome 10 by the polymerase chain reaction and fluorescence in situ hybridization". United States. https://doi.org/10.1016/0888-7543(95)80066-U.
@article{osti_250177,
title = {Localization of multiple human dihydrodiol dehydrogenase (DDH1 and DDH2) and chlordecone reductase (CHDR) genes in chromosome 10 by the polymerase chain reaction and fluorescence in situ hybridization},
author = {Khanna, M and Qin, K N and Belkin, S},
abstractNote = {Multiple human dihydrodiol dehydrogenases and human chlordecone reductase belong to the aldo-keto reductase superfamily. These two enzymes are involved in the metabolism of xenobiotics, such as polycyclic aromatic hydrocarbons and pesticides. Recently we have isolated three closely related genes encoding two dihydrodiol dehydrogenases (DDH1 and DDH2) and the chlordecone reductase (CHDR). Mapping of the location of the genes was performed using the polymerase chain reaction using gene-specific primers to amplify gene sequences in human/hamster hybrid DNA. All three genes were found to be located on chromosome 10. In situ hybridization using a lambda clone as the probe further confirmed regional localization at 10p14-p15. 13 refs., 2 figs.},
doi = {10.1016/0888-7543(95)80066-U},
url = {https://www.osti.gov/biblio/250177}, journal = {Genomics},
number = 2,
volume = 25,
place = {United States},
year = {Fri Jan 20 00:00:00 EST 1995},
month = {Fri Jan 20 00:00:00 EST 1995}
}