A boundary of long-range G+C% mosaic domains in the human MHC locus: Pseudoautosomal boundary-like sequence exists near the boundary
- Graduate Univ. for Advanced Studies, Mishima (Japan); and others
The human genome is composed of long-range G+C% (GC%) mosaic structures related to chromosome bands. We found the human MHC locus to be an example of megabase-level GC% mosaic structures and predicted a possible boundary of the megabase-level domains within an undercharacterized 450-kb region harboring the junction of MHC classes II and III. Chromosome walking of the 450-kb region and base-compositional analysis precisely located the boundary of the mosaic domains, disclosing a sharp GC% transition. Near the transition point there was a 20-kb dense Alu cluster, a 30-kb dense LINE-1 cluster, and a sequence highly homologous with the pseudoautosomal boundary of the short arms of human sex chromosomes (PAB1X and PAB1Y); PAB1X and PAB1Y are the interface between sex-specific and pseudoautosomal regions. Many PAB1XY-like sequences (PABLs) were detected by hybridization against genomic DNA, and the new sequences defined the complete form of PABLs to be about 650 nt. 49 refs., 5 figs., 2 tabs.
- OSTI ID:
- 241078
- Journal Information:
- Genomics, Vol. 25, Issue 1; Other Information: PBD: 1 Jan 1995
- Country of Publication:
- United States
- Language:
- English
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