Aberrant localization of lamin B receptor (LBR) in cellular senescence in human cells

Journal Article · Fri May 13 00:00:00 EDT 2016 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2016.04.018· OSTI ID:22598715

Arai, Rumi; En, Atsuki; Ukekawa, Ryo ^[1]; Miki, Kensuke ^[1]; Fujii, Michihiko ^[1]; Ayusawa, Dai ^[1]

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1. Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027 (Japan)

5-Bromodeoxyuridine (BrdU), a thymidine analogue, induces cellular senescence in mammalian cells. BrdU induces cellular senescence probably through the regulation of chromatin because BrdU destabilizes or disrupts nucleosome positioning and decondenses heterochromatin. Since heterochromatin is tethered to the nuclear periphery through the interaction with the nuclear envelope proteins, we examined the localization of the several nuclear envelope proteins such as lamins, lamin-interacting proteins, nuclear pore complex proteins, and nuclear transport proteins in senescent cells. We have shown here that lamin B receptor (LBR) showed a change in localization in both BrdU-induced and replicative senescent cells.

• OSTI ID: 22598715
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 473, Issue 4; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANIMAL CELLS
BUDR
HETEROCHROMATIN
NUCLEOSOMES
RECEPTORS
THYMIDINE