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Title: Cloning and characterization of a novel Gladiolus hybridus AFP family gene (GhAFP-like) related to corm dormancy

Abstract

Abscisic acid (ABA) is an important phytohormone controlling seed dormancy. AFPs (ABA INSENSITIVE FIVE BINDING PROTEINS) are reported to be negative regulators of the ABA signaling pathway. The involvement of AFPs in dormant vegetative organs remains poorly understood. Here, we isolated and characterized a novel AFP family member from Gladiolus dormant cormels, GhAFP-like, containing three conserved domains of the AFP family. Quantitative PCR analysis revealed that GhAFP-like was expressed in dormant organs and its expression was down-regulated along with corm storage. GhAFP-like was verified to be a nuclear-localized protein. Overexpressing GhAFP-like in Arabidopsis thaliana not only showed weaker seed dormancy with insensitivity to ABA, but also changed the expression of some ABA related genes. In addition, a primary root elongation assay showed GhAFP-like may involve in auxin signaling response. The results in this study indicate that GhAFP-like acts as a negative regulator in ABA signaling and is related to dormancy. - Highlights: • GhAFP-like is expessed in dormant corm. • Overexpressing GhAFP-like showed early germination and insensitivity to ABA. • Overexpressing GhAFP-like changed ABI5 downstream genes expression.

Authors:
;  [1];  [2];  [3]; ; ;  [1];  [1];  [1]
  1. Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100193 (China)
  2. Department of Biological Sciences, University of Toronto, Toronto, Ontario (Canada)
  3. College of Biology, Fuyang Normal College, Fuyang, Anhui (China)
Publication Date:
OSTI Identifier:
22594268
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 471; Journal Issue: 1; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ABSCISIC ACID; ARABIDOPSIS; AUXINS; CLONING; ELONGATION; GENES; GERMINATION; ORGANS; POLYMERASE CHAIN REACTION; PROTEINS; ROOTS; SEEDS

Citation Formats

Wu, Jian, Seng, Shanshan, Carianopol, Carina, Sui, Juanjuan, Yang, Qiuyan, Zhang, Fengqin, Jiang, Huiru, He, Junna, and Yi, Mingfang. Cloning and characterization of a novel Gladiolus hybridus AFP family gene (GhAFP-like) related to corm dormancy. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.01.146.
Wu, Jian, Seng, Shanshan, Carianopol, Carina, Sui, Juanjuan, Yang, Qiuyan, Zhang, Fengqin, Jiang, Huiru, He, Junna, & Yi, Mingfang. Cloning and characterization of a novel Gladiolus hybridus AFP family gene (GhAFP-like) related to corm dormancy. United States. https://doi.org/10.1016/J.BBRC.2016.01.146
Wu, Jian, Seng, Shanshan, Carianopol, Carina, Sui, Juanjuan, Yang, Qiuyan, Zhang, Fengqin, Jiang, Huiru, He, Junna, and Yi, Mingfang. 2016. "Cloning and characterization of a novel Gladiolus hybridus AFP family gene (GhAFP-like) related to corm dormancy". United States. https://doi.org/10.1016/J.BBRC.2016.01.146.
@article{osti_22594268,
title = {Cloning and characterization of a novel Gladiolus hybridus AFP family gene (GhAFP-like) related to corm dormancy},
author = {Wu, Jian and Seng, Shanshan and Carianopol, Carina and Sui, Juanjuan and Yang, Qiuyan and Zhang, Fengqin and Jiang, Huiru and He, Junna and Yi, Mingfang},
abstractNote = {Abscisic acid (ABA) is an important phytohormone controlling seed dormancy. AFPs (ABA INSENSITIVE FIVE BINDING PROTEINS) are reported to be negative regulators of the ABA signaling pathway. The involvement of AFPs in dormant vegetative organs remains poorly understood. Here, we isolated and characterized a novel AFP family member from Gladiolus dormant cormels, GhAFP-like, containing three conserved domains of the AFP family. Quantitative PCR analysis revealed that GhAFP-like was expressed in dormant organs and its expression was down-regulated along with corm storage. GhAFP-like was verified to be a nuclear-localized protein. Overexpressing GhAFP-like in Arabidopsis thaliana not only showed weaker seed dormancy with insensitivity to ABA, but also changed the expression of some ABA related genes. In addition, a primary root elongation assay showed GhAFP-like may involve in auxin signaling response. The results in this study indicate that GhAFP-like acts as a negative regulator in ABA signaling and is related to dormancy. - Highlights: • GhAFP-like is expessed in dormant corm. • Overexpressing GhAFP-like showed early germination and insensitivity to ABA. • Overexpressing GhAFP-like changed ABI5 downstream genes expression.},
doi = {10.1016/J.BBRC.2016.01.146},
url = {https://www.osti.gov/biblio/22594268}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 471,
place = {United States},
year = {Fri Feb 26 00:00:00 EST 2016},
month = {Fri Feb 26 00:00:00 EST 2016}
}