Daphnetin inhibits invasion and migration of LM8 murine osteosarcoma cells by decreasing RhoA and Cdc42 expression

Journal Article · Fri Feb 26 00:00:00 EST 2016 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2016.01.179· OSTI ID:22594260

Fukuda, Hiroki ^[1]; Nakamura, Seikou ^[2]; Chisaki, Yugo ^[3]; Takada, Tetsuya ^[1]; Toda, Yuki ^[4]; Murata, Hiroaki ^[5]; Itoh, Kazuyuki ^[6]; Yano, Yoshitaka ^[3]; Takata, Kazuyuki ^[1]; Ashihara, Eishi ^[1]

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1. Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University, Kyoto (Japan)
2. Department of Pharmacognosy, Kyoto Pharmaceutical University, Kyoto (Japan)
3. Education and Research Center for Clinical Pharmacy, Kyoto Pharmaceutical University, Kyoto (Japan)
4. Department of Medicinal Chemistry, Kyoto Pharmaceutical University, Kyoto (Japan)
5. Department of Orthopedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto (Japan)
6. Department of Biology, Osaka Medical Center of Cancer and Cardiovascular Diseases, Osaka (Japan)

Daphnetin, 7,8-dihydroxycoumarin, present in main constituents of Daphne odora var. marginatai, has multiple pharmacological activities including anti-proliferative effects in cancer cells. In this study, using a Transwell system, we showed that daphnetin inhibited invasion and migration of highly metastatic murine osteosarcoma LM8 cells in a dose-dependent manner. Following treatment by daphnetin, cells that penetrated the Transwell membrane were rounder than non-treated cells. Immunofluorescence analysis revealed that daphnetin decreased the numbers of intracellular stress fibers and filopodia. Moreover, daphnetin treatment dramatically decreased the expression levels of RhoA and Cdc42. In summary, the dihydroxycoumarin derivative daphnetin inhibits the invasion and migration of LM8 cells, and therefore represents a promising agent for use against metastatic cancer. - Highlights: • Daphnetin, a coumarin-derivative, inhibited invasion and migration of LM8 cells. • Stress fibers and filopodia were decreased by daphnetin treatment. • Daphnetin decreased RhoA and Cdc42 protein expression.

• OSTI ID: 22594260
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 471, Issue 1; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BIOLOGICAL STRESS
CALVES
COUMARIN
DMSO
FIBERS
GUANIDINES
MEMBRANES
METASTASES
OSTEOSARCOMAS
PHOSPHATES
PROTEINS
RIBOSOMAL RNA