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Title: WE-EF-BRA-01: A Dual-Use Optical Tomography System for Small Animal Radiation Research Platform (SARRP)

Abstract

Purpose: We develop a novel dual-use configuration for a tri-modality, CBCT/bioluminescence tomography(BLT)/fluorescence tomography(FT), imaging system with the SARRP that can function as a standalone system for longitudinal imaging research and on-board the SARRP to guide irradiation. BLT provides radiation guidance for soft tissue target, while FT offers functional information allowing mechanistic investigations. Methods: The optical assembly includes CCD camera, lens, filter wheel, 3-way mirrors, scanning fiber system and light-tight enclosure. The rotating mirror system directs the optical signal from the animal surface to the camera at multiple projection over 180 degree. The fiber-laser system serves as the external light source for the FT application. Multiple filters are used for multispectral imaging to enhance localization accuracy using BLT. SARRP CBCT provides anatomical information and geometric mesh for BLT/FT reconstruction. To facilitate dual use, the 3-way mirror system is cantilevered in front of the camera. The entire optical assembly is driven by a 1D linear stage to dock onto an independent mouse support bed for standalone application. After completion of on-board optical imaging, the system is retracted from the SARRP to allow irradiation of the mouse. Results: A tissue-simulating phantom and a mouse model with a luminescence light source are used tomore » demonstrate the function of the dual-use optical system. Feasibility data have been obtained based on a manual-docking prototype. The center of mass of light source determined in living mouse with on-board BLT is within 1±0.2mm of that with CBCT. The performance of the motorized system is expected to be the same and will be presented. Conclusion: We anticipate the motorized dual use system provide significant efficiency gain over our manual docking and off-line system. By also supporting off-line longitudinal studies independent of the SARRP, the dual-use system is a highly efficient and cost-effective platform to facilitate optical imaging for pre-clinical radiation research. The work is supported by NIH R01CA158100 and Xstrahl Ltd. Drs. John Wong and Iulian Iordachita receive royalty payment from a licensing agreement between Xstrahl Ltd and Johns Hopkins University. John Wong also has a consultant agreement with Xstrahl Ltd.« less

Authors:
; ;  [1]; ;  [2]
  1. Department of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins Hospital, Baltimore, MD (United States)
  2. Laboratory for Computational Sensing and Robotics, Johns Hopkins University, Baltimore, MD (United States)
Publication Date:
OSTI Identifier:
22572200
Resource Type:
Journal Article
Journal Name:
Medical Physics
Additional Journal Information:
Journal Volume: 42; Journal Issue: 6; Other Information: (c) 2015 American Association of Physicists in Medicine; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0094-2405
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 61 RADIATION PROTECTION AND DOSIMETRY; ACCURACY; ANIMAL TISSUES; BIOLUMINESCENCE; BIOMEDICAL RADIOGRAPHY; CAMERAS; CHARGE-COUPLED DEVICES; COMPUTERIZED TOMOGRAPHY; FLUORESCENCE; LIGHT SOURCES; MIRRORS; OPTICAL SYSTEMS; PHANTOMS

Citation Formats

Wang, K, Bin, Z, Wong, J, He, X, and Iordachita, I. WE-EF-BRA-01: A Dual-Use Optical Tomography System for Small Animal Radiation Research Platform (SARRP). United States: N. p., 2015. Web. doi:10.1118/1.4925980.
Wang, K, Bin, Z, Wong, J, He, X, & Iordachita, I. WE-EF-BRA-01: A Dual-Use Optical Tomography System for Small Animal Radiation Research Platform (SARRP). United States. https://doi.org/10.1118/1.4925980
Wang, K, Bin, Z, Wong, J, He, X, and Iordachita, I. 2015. "WE-EF-BRA-01: A Dual-Use Optical Tomography System for Small Animal Radiation Research Platform (SARRP)". United States. https://doi.org/10.1118/1.4925980.
@article{osti_22572200,
title = {WE-EF-BRA-01: A Dual-Use Optical Tomography System for Small Animal Radiation Research Platform (SARRP)},
author = {Wang, K and Bin, Z and Wong, J and He, X and Iordachita, I},
abstractNote = {Purpose: We develop a novel dual-use configuration for a tri-modality, CBCT/bioluminescence tomography(BLT)/fluorescence tomography(FT), imaging system with the SARRP that can function as a standalone system for longitudinal imaging research and on-board the SARRP to guide irradiation. BLT provides radiation guidance for soft tissue target, while FT offers functional information allowing mechanistic investigations. Methods: The optical assembly includes CCD camera, lens, filter wheel, 3-way mirrors, scanning fiber system and light-tight enclosure. The rotating mirror system directs the optical signal from the animal surface to the camera at multiple projection over 180 degree. The fiber-laser system serves as the external light source for the FT application. Multiple filters are used for multispectral imaging to enhance localization accuracy using BLT. SARRP CBCT provides anatomical information and geometric mesh for BLT/FT reconstruction. To facilitate dual use, the 3-way mirror system is cantilevered in front of the camera. The entire optical assembly is driven by a 1D linear stage to dock onto an independent mouse support bed for standalone application. After completion of on-board optical imaging, the system is retracted from the SARRP to allow irradiation of the mouse. Results: A tissue-simulating phantom and a mouse model with a luminescence light source are used to demonstrate the function of the dual-use optical system. Feasibility data have been obtained based on a manual-docking prototype. The center of mass of light source determined in living mouse with on-board BLT is within 1±0.2mm of that with CBCT. The performance of the motorized system is expected to be the same and will be presented. Conclusion: We anticipate the motorized dual use system provide significant efficiency gain over our manual docking and off-line system. By also supporting off-line longitudinal studies independent of the SARRP, the dual-use system is a highly efficient and cost-effective platform to facilitate optical imaging for pre-clinical radiation research. The work is supported by NIH R01CA158100 and Xstrahl Ltd. Drs. John Wong and Iulian Iordachita receive royalty payment from a licensing agreement between Xstrahl Ltd and Johns Hopkins University. John Wong also has a consultant agreement with Xstrahl Ltd.},
doi = {10.1118/1.4925980},
url = {https://www.osti.gov/biblio/22572200}, journal = {Medical Physics},
issn = {0094-2405},
number = 6,
volume = 42,
place = {United States},
year = {Mon Jun 15 00:00:00 EDT 2015},
month = {Mon Jun 15 00:00:00 EDT 2015}
}