Copper accumulation in channel catfish (Ictalurus punctatus) exposed to water borne copper sulfate
- Oak Ridge Inst. for Science and Education, TN (United States)
- National Biological Service, Stuttgart, AR (United States)
- Univ. of Mississippi, Oxford, MS (United States)
- National Center for Toxicological Research, Jefferson, AR (United States)
Liver and axial muscle of channel catfish (Ictalurus punctatus) was analyzed for residual copper after exposure to water borne copper sulfate. Copper sulfate was continuously introduced into well water in three fiber glass tanks to achieve 1.7 mg/L, 2.7 mg/L and 3.6 mg/L copper sulfate concentrations in exposure waters. Milli-Q quality water was metered into a fourth tank at the same rate for unexposed fish. Actual levels of copper in exposure waters were determined by daily sampling and analysis by graphite furnace atomic absorption spectrophotometry (GFAA). Tissue samples were taken from six fish from each of the exposed and unexposed tanks at two-week intervals, Samples were collected until tissue analysis indicated an equilibrium had been established between the uptake and elimination in both the muscle and liver tissue. Elimination was followed until a clear rate of deputation could be established. Samples were digested in nitric acid in a micro wave digestor and analyzed by GFAA. Results of tissue analysis will be presented to demonstrate bioaccumulation and the effect of copper concentration, length of copper exposure, and gender on copper uptake, establishment of tissue:environmental copper equilibrium, and rate of copper elimination following exposure.
- OSTI ID:
- 225201
- Report Number(s):
- CONF-9511137-; ISBN 1-880611-03-1; TRN: IM9621%%131
- Resource Relation:
- Conference: 2. Society of Environmental Toxicology and Chemistry (SETAC) world conference, Vancouver (Canada), 5-9 Nov 1995; Other Information: PBD: 1995; Related Information: Is Part Of Second SETAC world congress (16. annual meeting): Abstract book. Global environmental protection: Science, politics, and common sense; PB: 378 p.
- Country of Publication:
- United States
- Language:
- English
Similar Records
Metallothionein (MT) induction in channel catfish following treatment with therapeutic levels of copper sulfate
Static bioassay testing of Iowa Coal Beneficiation Plant magnetite-slurry with channel catfish (Ictalurus punctatus)