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Title: Applications of thin-film sandwich crystallization platforms

Crystallization via sandwiches of thin polymer films is presented and discussed. Examples are shown of protein crystallization in, and data collection from, solutions sandwiched between thin polymer films using vapour-diffusion and batch methods. The crystallization platform is optimal for both visualization and in situ data collection, with the need for traditional harvesting being eliminated. In wells constructed from the thinnest plastic and with a minimum of aqueous liquid, flash-cooling to 100 K is possible without significant ice formation and without any degradation in crystal quality. The approach is simple; it utilizes low-cost consumables but yields high-quality data with minimal sample intervention and, with the very low levels of background X-ray scatter that are observed, is optimal for microcrystals.
Authors:
; ; ;  [1]
  1. Diamond Light Source, Harwell Oxford, Didcot OX11 0DE (United Kingdom)
Publication Date:
OSTI Identifier:
22515184
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F, Structural Biology Communications; Journal Volume: 72; Journal Issue: Pt 4; Other Information: PMCID: PMC4822989; PMID: 27050266; PUBLISHER-ID: nj5248; PUBLISHER-ID: S2053230X16004386; OAI: oai:pubmedcentral.nih.gov:4822989; Copyright (c) Axford et al. 2016; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; CRYSTALS; MATHEMATICAL SOLUTIONS; THIN FILMS; X RADIATION