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Title: Crystallization and X-ray diffraction studies of a complete bacterial fatty-acid synthase type I

Bacterial and fungal type I fatty-acid synthases (FAS I) are evolutionarily connected, as bacterial FAS I is considered to be the ancestor of fungal FAS I. In this work, the production, crystallization and X-ray diffraction data analysis of a bacterial FAS I are reported. While a deep understanding of the fungal and mammalian multi-enzyme type I fatty-acid synthases (FAS I) has been achieved in recent years, the bacterial FAS I family, which is narrowly distributed within the Actinomycetales genera Mycobacterium, Corynebacterium and Nocardia, is still poorly understood. This is of particular relevance for two reasons: (i) although homologous to fungal FAS I, cryo-electron microscopic studies have shown that bacterial FAS I has unique structural and functional properties, and (ii) M. tuberculosis FAS I is a drug target for the therapeutic treatment of tuberculosis (TB) and therefore is of extraordinary importance as a drug target. Crystals of FAS I from C. efficiens, a homologue of M. tuberculosis FAS I, were produced and diffracted X-rays to about 4.5 Å resolution.
Authors:
 [1] ;  [2] ;  [3] ;  [1] ;  [1] ;  [2]
  1. Goethe University Frankfurt, Max-von-Laue-Strasse 15, 60438 Frankfurt am Main (Germany)
  2. (Germany)
  3. EMBL Grenoble, 71 Avenue des Martyrs, 38042 Grenoble CEDEX 9 (France)
Publication Date:
OSTI Identifier:
22515176
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F, Structural Biology Communications; Journal Volume: 71; Journal Issue: Pt 11; Other Information: PMCID: PMC4631590; PMID: 26527268; PUBLISHER-ID: no5090; PUBLISHER-ID: S2053230X15018336; OAI: oai:pubmedcentral.nih.gov:4631590; Copyright (c) Enderle et al. 2015; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; DATA ANALYSIS; X RADIATION; X-RAY DIFFRACTION