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Title: Morphological and molecular characterization of fungal pathogen, Magnaphorthe oryzae

Rice is arguably the most crucial food crops supplying quarter of calories intake. Fungal pathogen, Magnaphorthe oryzae promotes blast disease unconditionally to gramineous host including rice species. This disease spurred an outbreaks and constant threat to cereal production. Global rice yield declining almost 10-30% including Malaysia. As Magnaphorthe oryzae and its host is model in disease plant study, the rice blast pathosystem has been the subject of intense interest to overcome the importance of the disease to world agriculture. Therefore, in this study, our prime objective was to isolate samples of Magnaphorthe oryzae from diseased leaf obtained from MARDI Seberang Perai, Penang, Malaysia. Molecular identification was performed by sequences analysis from internal transcribed spacer (ITS) region of nuclear ribosomal RNA genes. Phylogenetic affiliation of the isolated samples were analyzed by comparing the ITS sequences with those deposited in the GenBank database. The sequence of the isolate demonstrated at least 99% nucleotide identity with the corresponding sequence in GenBank for Magnaphorthe oryzae. Morphological observed under microscope demonstrated that the structure of conidia followed similar characteristic as M. oryzae. Finding in this study provide useful information for breeding programs, epidemiology studies and improved disease management.
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [5] ;  [6] ;  [7]
  1. Faculty of Applied Science, UniversitiTeknologi MARA (UiTM), Kuala Pilah, Negeri Sembilan (Malaysia)
  2. Institute of Tropical Agriculture, Universiti Putra Malaysia (UPM), Serdang, Selangor (Malaysia)
  3. (UPM), Serdang, Selangor (Malaysia)
  4. Agrotechnology & Bioscience Division, Malaysian Nuclear Agency, Bangi, Kajang, Selangor (Malaysia)
  5. Department of Plant Protection, Universiti Putra Malaysia (UPM), Serdang, Selangor (Malaysia)
  6. Department of Agriculture Technology, Universiti Putra Malaysia (UPM), Serdang, Selangor (Malaysia)
  7. Faculty of Plantation and Agrotechnology, UniversitiTeknologi MARA (UiTM), Shah Alam, Selangor (Malaysia)
Publication Date:
OSTI Identifier:
22494568
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 1705; Journal Issue: 1; Conference: 1. conference on progress in applied mathematics in science and engineering (PIAMSE) 2015, Bali (Indonesia), 29 Sep - 1 Oct 2015; Other Information: (c) 2016 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AGRICULTURE; CONIDIA; CROPS; DISEASES; EPIDEMIOLOGY; GENES; HOST; INTAKE; LEAVES; MALAYSIA; NUCLEOTIDES; PATHOGENS; RIBOSOMAL RNA; RICE; STRUCTURAL CHEMICAL ANALYSIS