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Title: Repeatability and reproducibility of intracellular molar concentration assessed by synchrotron-based x-ray fluorescence microscopy

Elemental analysis of biological sample can give information about content and distribution of elements essential for human life or trace elements whose absence is the cause of abnormal biological function or development. However, biological systems contain an ensemble of cells with heterogeneous chemistry and elemental content; therefore, accurate characterization of samples with high cellular heterogeneity may only be achieved by analyzing single cells. Powerful methods in molecular biology are abundant, among them X-Ray microscopy based on synchrotron light source has gaining increasing attention thanks to its extremely sensitivity. However, reproducibility and repeatability of these measurements is one of the major obstacles in achieving a statistical significance in single cells population analysis. In this study, we compared the elemental content of human colon adenocarcinoma cells obtained by three distinct accesses to synchrotron radiation light.
Authors:
;  [1] ; ; ; ; ;  [2] ;  [3] ;  [4] ;  [5] ;  [6] ;  [7] ;  [4] ;  [2] ;  [4]
  1. Elettra - Sincrotrone Trieste, 34149 Basovizza, Trieste (Italy)
  2. Department of Pharmacy and Biotechnology, University of Bologna, Bologna 40127 (Italy)
  3. Museo Storico della Fisica e Centro Studi e Ricerche Enrico Fermi, Piazza del Viminale 1, 00184 Roma Italy (Italy)
  4. (Italy)
  5. Institute for Photonics and Nanotechnology, Consiglio Nazionale delle Richerche, 00156 Rome (Italy)
  6. Department of Chemistry “G. Ciamician”, University of Bologna, Bologna 40126 (Italy)
  7. Institute of Chemical-Physical Processes, Sapienza University of Rome, 00185 Rome (Italy)
Publication Date:
OSTI Identifier:
22494396
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 1696; Journal Issue: 1; Conference: XRM 2014: 12. international conference on X-ray microscopy, Melbourne (Australia), 26-31 Oct 2014; Other Information: (c) 2016 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; 71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; AUGMENTATION; BIOLOGICAL FUNCTIONS; CARCINOMAS; CHEMISTRY; COMPARATIVE EVALUATIONS; CONCENTRATION RATIO; FLUORESCENCE; HUMAN POPULATIONS; LARGE INTESTINE; LIGHT SOURCES; MICROSCOPY; MOLECULAR BIOLOGY; SENSITIVITY; SYNCHROTRON RADIATION; SYNCHROTRONS; TRACE AMOUNTS; X RADIATION