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Title: 2D/3D cryo x-ray fluorescence imaging at the bionanoprobe at the advanced photon source

Trace elements, particularly metals, play very important roles in biological systems. Synchrotron-based hard X-ray fluorescence microscopy offers the most suitable capabilities to quantitatively study trace metals in thick biological samples, such as whole cells and tissues. In this manuscript, we have demonstrated X-ray fluorescence imaging of frozen-hydrated whole cells using the recent developed Bionanoprobe (BNP). The BNP provides spatial resolution down to 30 nm and cryogenic capabilities. Frozen-hydrated biological cells have been directly examined on a sub-cellular level at liquid nitrogen temperatures with minimal sample preparation.
Authors:
; ;  [1] ; ; ;  [2] ;  [3] ; ;  [4] ; ;  [5] ;  [6] ;  [1] ;  [7] ;  [7] ;  [7] ;  [1] ;  [7]
  1. Advanced Photon Source, Argonne National Laboratory, Lemont, IL 60439 (United States)
  2. Department of Radiation Oncology, Northwester University, Chicago, IL 60611 (United States)
  3. Applied Physics, Northwestern University, Evanston, IL 60208 (United States)
  4. Department of Physics and Astronomy, Northwestern University, Evanston, IL 60208 (United States)
  5. Carl Zeiss X-ray Microscopy, Pleasanton, CA 94588 (United States)
  6. Synchrotron Research Center, Northwestern University, Argonne, IL 60439 (United States)
  7. (United States)
Publication Date:
OSTI Identifier:
22494393
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 1696; Journal Issue: 1; Conference: XRM 2014: 12. international conference on X-ray microscopy, Melbourne (Australia), 26-31 Oct 2014; Other Information: (c) 2016 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; 71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; ADVANCED PHOTON SOURCE; ANIMAL TISSUES; FLUORESCENCE; HARD X RADIATION; IMAGES; LIQUEFIED GASES; METALS; MICROSCOPY; NITROGEN; SAMPLE PREPARATION; SPATIAL RESOLUTION; SYNCHROTRONS; TRACE AMOUNTS